Affinity adsorbents for fibrinogen
For the separation, removal, isolation, purification, characterisation, identification or quantification of fibrinogen or a protein that is a fibrinogen analogue, an affinity adsorbent is used that is a compound of formula II wherein one X is N and the other is N, C—CI or C—CN; Y is O, S or NR 2 ; Z is O, S or NR 3 ; R 2 and R 3 are each H, alkyl, hydroxyalkyl, benzyl or β-phenylethyl; n is 0 to 6; A is a support matrix, optionally linked to the triazine ring by a spacer; R 7 is a group bearing a positive charge at neutral pH; W is an optional linker; V is an aromatic group; and R 8 and R 9 are each H, OH, alkyl, alkoxy, amino, NH 2 , acyloxy, acylamino, CO 2 H, sulphonic acid, carbamoyl, sulphamoyl, alkylsulphonyl or halogen or a cyclic structure such as a morpholino group, or R 8 and R 9 are linked to form such a cyclic structure.
1. A novel compound which is of formula II:
wherein one X is N and the other is N, C—Cl or C—CN;
Y is O, S or NR 2 ;
R 2 and R 3 are each H, alkyl, hydroxyalkyl, benzyl or β-phenylethyl;
n is 0 to 6;
A is a support matrix, selected from agarose, silica, cellulose, dextran, starch, alginate, carrageenan, synthetic polymers, glass and metal oxides, wherein A is optionally linked to the triazine ring by a spacer;
R 7 is a group bearing a positive charge at neutral pH;
W is an optional linker;
either V is an aromatic group; R 8 is H, OH, alkyl, alkoxy, amino, acyloxy, acylamino, CO 2 H, sulphonic acid, carbamoyl, sulphamoyl, alkylsulphonyl or halogen and R 9 is a morpholino group, or
VR 8 R 9 is morphine.
2. The compound, according to claim 1 , wherein VR 8 R 9 is morpholine.
3. A compound of formula III:
4. A compound of formula IV:
5. A compound of formula V:
6. A compound of formula VI:
7. A compound of formula VII: