Compositions and methods of selective nucleic acid isolation
The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
1. A method for identifying DNA and RNA in a biological sample comprising:
selectively binding DNA to a first solid phase by contacting the biological sample with the first solid phase under conditions which selectively bind DNA;
separating the first solid phase with the bound DNA from a first unbound portion of the biological sample;
identifying the DNA bound to the first solid phase; and
identifying the RNA from the first unbound portion of the biological sample.
2. The method of claim 1 , wherein the identifying the DNA bound to the first solid phase comprises amplifying the DNA bound to the first solid phase.
3. The method of claim 1 , wherein the conditions which selectively bind DNA comprise using a binding buffer comprising picrate, tannate, tungstate, molybdate, perchlorate, sulfosalicylate, trichloroacetate, tribromoacetate, thiocyanate, nitrate, iodide or bromide.
4. The method of claim 1 , wherein the conditions which selectively bind DNA comprise using a binding buffer comprising trichloroacetate, tribromoacetate, thiocyanate, or nitrate.
5. The method of claim 1 , wherein the conditions which selectively bind DNA comprise using a binding buffer comprising thiocyanate, iodide or bromide.
6. The method of claim 1 , wherein the conditions which selectively bind DNA comprise using a binding buffer comprising thiocyanate.
7. The method of claim 1 , wherein the DNA is genomic DNA.
8. The method of claim 1 , wherein the DNA is genomic DNA and the conditions which selectively bind genomic DNA comprise using a binding buffer comprising thiocyanate, or iodide.
9. The method of claim 1 , wherein the DNA is genomic DNA and the conditions which selectively bind genomic DNA comprise using a binding buffer comprising thiocyanate.
10. The method of claim 7 , wherein identifying the genomic DNA bound to the first solid phase comprises hybridization to labeled probes, mass spectrometry, or detection by a reaction of the bound DNA with a label.
11. The method of claim 10 wherein detection by a reaction of the bound genomic DNA with a label comprises detection of fluorescence following the addition of a DNA-binding fluorophore.
12. The method of claim 7 , wherein identifying the genomic DNA bound to the first solid phase comprises hybridization to labeled probes.
13. The method of claim 1 , wherein the solid phase is in a form selected from a group comprising a particle, a bead, a membrane, and a frit.