Detection, prevention, and treatment of anthrax and other infectious diseases
Compositions and methods for the detection, prevention, or treatment of anthrax or other infectious diseases. In one aspect, the present invention provides methods for immunizing humans or animals against Bacillus anthracis or other capsulated pathogens. The methods include administering a capsular polypeptide of a pathogen of interest and a CD40 agonist to a human or animal. The capsular polypeptide or the CD40 agonist is administered in such an amount or frequency that an immunoprotective response can be elicited in the human or animal against the pathogen of interest. In another aspect, the present invention provides methods of using passive immunization with anti-capsular polypeptide antibodies to prevent or treat infections caused by Bacillus anthracis or other pathogens. In yet another aspect, the present invention provides methods useful for diagnosis of anthrax by detection of capsular polypeptide in serum or other biological samples.
1. A method of diagnosing infection by a PGA producing pathogen in a subject from a vertebrate species, comprising:
assaying soluble PGA in a biological sample obtained from the subject using an anti-PGA antibody, the assay generating a detectable signal if the level of soluble PGA in the sample exceeds a predetermined amount, the predetermined amount is 140 pg/ml.
2. The method of claim 1 wherein the subject is a human.
3. The method of claim 1 , further comprising administering antibiotics to the subject if the soluble PGA in the sample exceeds the predetermined amount.
4. The method of claim 1 , wherein assaying the soluble PGA in a biological sample comprises subjecting the sample to a lateral flow assay.
5. The method of claim 4 , wherein the lateral flow assay comprises the anti-PGA antibody coupled to a colloidal metal.
6. The method of claim 1 , wherein the anti-PGA antibody comprises an IgG3 isotype anti-PGA antibody.
7. The method of claim 1 , wherein the anti-PGA antibody is from a species other than the species of the subject.
8. The method of claim 1 , wherein the anti-PGA antibody is obtained by immunizing a vertebrate with PGA under conditions of enhanced immunogenicity.
9. The method of claim 8 , further comprising isolating the antibody for use in the assay.
10. The method of claim 1 , wherein the perceptible indication comprises a visually perceptible indication.
11. A method of diagnosing a subject as being infected by a PGA producing pathogen, comprising detecting soluble PGA exceeding a predetermined amount, the predetermined amount is 140 pg/ml, in a sample obtained from a subject known to be at risk for acquiring anthrax.
12. The method of claim 1 , wherein the detectable signal is a visual indicator.
13. The method of claim 11 , further comprising administering antibiotics to the subject if the soluble PGA in the sample exceeds the predetermined amount.