Blood storage bag system and depletion devices with oxygen and carbon dioxide depletion capabilities
A blood storage system. The system has a collection bag for red blood cells; an oxygen/carbon dioxide depletion device; a storage bag for red blood cells; and tubing connecting the collection bag to the depletion device and the depletion device to the storage bag. The depletion device includes a receptacle of a solid material having an inlet and an outlet adapted to receiving and expelling a flushing gas; a plurality of hollow fibers or gas-permeable films extending within the receptacle from an entrance to an exit thereof. The hollow fibers or gas-permeable films are adapted to receiving and conveying red blood cells.
1. A method for removing oxygen from red blood cells, comprising:
passing red blood cells through an oxygen depletion device, wherein said depletion device comprises:
a receptacle of a solid material having an inlet and an outlet adapted to receiving and expelling a flushing gas; and
a plurality of gas-permeable films or membranes extending within said receptacle from an entrance to an exit thereof, wherein said plurality of gas-permeable films or membranes are formed of a material permeable to oxygen and are adapted to receiving and conveying said red blood cells,
wherein said plurality of gas-permeable films or membranes are each constructed in a flat sheet, and wherein said flushing gas comprises 5% CO 2 .
2. The blood storage system comprising,
a collection bag for red blood cells;
an oxygen depletion device;
an oxygen impermeable storage bag for red blood cells; and
tubing connecting said collection bag to said oxygen depletion device and tubing connecting said oxygen depletion device to said oxygen impermeable storage bag,
wherein said oxygen depletion device comprises:
a) a cartridge;
a plurality of gas-permeable films or membranes formed of a material permeable to oxygen and are adapted to receiving and conveying red blood cells extending within said cartridge from an entrance to an exit thereof; and
an amount of an oxygen scavenger packed within said cartridge and contiguous to and in between said plurality of gas-permeable films or membranes; or,
b) a receptacle of a solid material having an inlet and an outlet adapted to receiving and expelling a flushing gas; and
a plurality of gas-permeable films or membranes formed of a material permeable to oxygen and are adapted to receiving and conveying red blood cells extending within said receptacle from an entrance to an exit thereof,
wherein said plurality of gas-permeable films or membranes are each constructed in a flat sheet, and wherein said oxygen impermeable storage bag for red blood cells has an inner blood-compatible surface comprising di-2-ethylhexyl phthalate (DEHP) plasticized polyvinyl-chlorine (PVC).
3. A blood storage system comprising:
a collection bag for red blood cells;
a unitary device for depleting oxygen and reducing leukocytes from red blood cells;
an oxygen impermeable storage bag for red blood cells; and
tubing connecting the collection bag to said unitary device and tubing connecting said unitary device to said oxygen impermeable storage bag.
4. The blood storage system of claim 3 , wherein said unitary device further comprises platelet depletion.
5. The blood storage system of claim 3 , wherein said oxygen impermeable storage bag for red blood cells has an inner blood-compatible surface comprising di-2-ethylhexyl phthalate (DEHP) plasticized polyvinyl-chlorine (PVC).
6. The blood storage system of claim 3 , wherein said oxygen impermeable storage bag for red blood cells has an inner blood-compatible surface without di-2-ethylhexyl phthalate (DEHP).
7. A method for increasing adenosine triphosphate (ATP) levels in red blood cells, comprising: mixing red blood cells with an acidic additive solution, and passing said red blood cells through an oxygen depletion device,
wherein said oxygen depletion device comprises:
a) a cartridge;
a plurality of gas-permeable films or membranes, extending within said cartridge from an entrance to an exit thereof, formed of a material permeable to oxygen and adapted to receiving and conveying said red blood cells; and
an amount of an oxygen scavenger packed within said cartridge and contiguous to and in between said plurality of gas-permeable films or membranes; or,
b) a receptacle of a solid material having an inlet and an outlet adapted to receiving and expelling a flushing gas; and
a plurality of gas-permeable films or membranes formed of a material permeable to oxygen and are adapted to receiving and conveying said red blood cells extending within said receptacle from an entrance to an exit thereof,
wherein said plurality of gas-permeable films or membranes are each constructed in a flat sheet.
8. The method of claim 7 , wherein said oxygen depletion device depletes oxygen in said red blood cells to less than, or equal to, 4% oxygen saturation (SO 2 ).