Concentrating components of fluid circulated through a cell growth chamber
One or more embodiments are described directed to a method and system for concentrating components of a fluid circulated through a cell growth chamber such as a cell growth chamber. Accordingly, embodiments include methods and systems that utilize a tangential flow filter to concentrate components of a fluid that in embodiments includes expanded cells. In embodiments, a concentrated fluid component and a concentrated cell component are generated by flowing the fluid with expanded cells across a tangential flow filter. The concentrated cell component may be recirculated to the tangential flow filter to reach some desired concentration of cells. The concentrated fluid component may be collected to utilize cellular-produced constituents in the concentrated fluid component.
1. A method of concentrating a cellular component from a fluid circulated in a cell growth chamber, the method comprising:
expanding cells within a cell growth chamber, wherein the cell growth chamber comprises a hollow fiber membrane with an intracapillary space and an extracapillary space, wherein the expanding further comprises:
circulating, with a first pump, fluid in the intracapillary space of the cell growth chamber, the fluid comprising nutrients that promote cell growth;
expanding the cells in the intracapillary space;
circulating, with a second pump, media within the extracapillary space and through an oxygenator to provide oxygen to the cells;
removing expanded cells from the cell growth chamber using the fluid;
circulating, with the first pump, the fluid with the expanded cells across a tangential flow filter that is fluidly associated with the cell growth chamber, as part of a closed system, to generate a concentrated fluid component and a concentrated cell component, wherein the fluid flows out of a port of the cell growth chamber and directly into the tangential flow filter without exposure to the ambient environment;
recirculating, with the first pump, the concentrated cell component across the tangential flow filter to further concentrate the cells in the concentrated cell component;
collecting at least a portion of the concentrated fluid component in a container; and
collecting at least a portion of the further concentrated cellular component in a container.
2. The method of claim 1 , further comprising:
before circulating the fluid across the tangential flow filter, adding a second fluid to the fluid.
3. The method of claim 1 , further comprising:
before recirculating the concentrated cell component across the tangential flow filter, adding a second fluid to the concentrated cell component.
4. The method of claim 1 , wherein the recirculating is performed until a predetermined concentration of cells in the concentrated cell component is reached.
5. The method of claim 1 further comprising:
introducing the concentrated cell component back into the cell growth chamber.