Methods and compositions for targeting progenitor cell lines
The invention provides methods, compositions and kits for the identification and enrichment of progenitor cell lines obtained from pluripotent stem cells.
1. A peptide that binds to a progenitor cell wherein the peptide is selected from the group consisting of peptides comprising the following sequences: SWTYSYPNQNMD (SEQ ID NO: 1); DWTYSLPGLVEE (SEQ ID NO: 2); NWTWSMPTGNPA (SEQ ID NO: 3); GMTLRVLTN-YTE- (SEQ ID NO: 4); TLHVSENSWTYN (SEQ ID NO: 5); DWLWSFAPNVDT (SEQ ID NO: 6); TLSSQNPYMHKK (SEQ ID NO: 7); IDKQMMTSHKAI (SEQ ID NO: 8); QGMETQKLRMLK (SEQ ID NO: 9); GWYWETPLDMFN (SEQ ID NO: 10); GWVIDYDYYPMR (SEQ ID NO: 11); VTAENYQSFSVS (SEQ ID NO: 12); NNKMDDRMMMSIV (SEQ ID NO: 13); STGTDLHSNARI (SEQ ID NO: 14); YEFDNLLNRTLW (SEQ ID NO: 15); and EWTVNERTMWDL (SEQ ID NO: 16).
2. The peptide of claim 1 , wherein the peptide has plexin homology.
3. A composition comprising a progenitor cell and a peptide specifically bound to the progenitor cell, wherein the progenitor cell is the in vitro progeny of a pluripotent stem cell, and wherein the peptide has a length of 8-35 amino acids.
4. The composition of claim 3 , wherein the progenitor cell is a human progenitor cell.
5. The composition of claim 4 , wherein the progenitor cell is an endoderm progenitor cell.
6. The composition of claim 4 , wherein the progenitor cell is a mesoderm progenitor cell.
7. The composition of claim 3 , wherein the progenitor cell is the W10 progenitor cell.
8. The composition of claim 3 , wherein the progenitor cell expresses the markers heart and neural crest derivatives-expressed 2 (HAND2), HOXA4 and HOXB7.
9. The composition of claim 3 , wherein the peptide is selected from the group consisting of peptides comprising the following sequences: SWTYSYPNQNMD (SEQ ID NO: 1); DWTYSLPGLVEE (SEQ ID NO: 2); NWTWSMPTGNPA (SEQ ID NO: 3); GMTLRVLTN-YTE- (SEQ ID NO: 4); TLHVSENSWTYN (SEQ ID NO: 5); DWLWSFAPNVDT (SEQ ID NO: 6); TLSSQNPYMHKK (SEQ ID NO: 7); IDKQMMTSHKAI (SEQ ID NO: 8); QGMETQKLRMLK (SEQ ID NO: 9); GWYWETPLDMFN (SEQ ID NO: 10); GWVIDYDYYPMR (SEQ ID NO: 11); VTAENYQSFSVS (SEQ ID NO: 12); NNKMDDRMMMSIV (SEQ ID NO: 13); STGTDLHSNARI (SEQ ID NO: 14); YEFDNLLNRTLW (SEQ ID NO: 15); and EWTVNERTMWDL (SEQ ID NO: 16).
10. The composition of claim 3 , wherein the peptide has plexin homology.
11. The composition of claim 3 , wherein the peptide comprises a detectable substance.
12. The composition of claim 11 , wherein the detectable substance is an amino acid sequence.