Connector compound peptide phage display
The invention relates to a complex comprising a phage particle, said phage particle comprising (i) a polypeptide; (ii) a nucleic acid encoding the polypeptide of (i); (iii) a connector compound attached to said polypeptide wherein said connector compound is attached to the polypeptide by at least three discrete covalent bonds. The invention also relates to libraries, and to methods for making complexes and to methods of screening using same.
1. A method for making a complex, said method comprising
(a) displaying one or more polypeptides in a phage display system, wherein the polypeptide comprises the sequence (X)IC(X)mC(X)nC(X)o (SEQ ID NO. 67), wherein C represents the amino acid cysteine, X represents a random amino acid, m and n are numbers between 1 and 20 defining the length of intervening polypeptide segments and I and o are numbers between 0 and 20 defining the length of the flanking polypeptide segments;
(b) reducing the cysteine amino acids of the displayed polypeptides;
(c) the polypeptide comprising reduced cysteine amino acids is purified by filtration;
(d) providing tris-(bromomethyl)benzene (TBMB); and
(e) attaching TBMB to said reduced and purified polypeptide by formation of three discrete covalent bonds between said TBMB and said polypeptide via said cysteine amino acid residues, and form at least one loop which comprises a sequence of two or more amino acids subtended between two of said cysteine amino acid residues, thus forming the complex.
2. A method according to claim 1 , wherein following the filtration purification step (c), the polypeptide is maintained in the reduced state for bonding to the connector compound by incubation in degassed buffer and in the presence of chelating agent.
3. A method according to claim 1 , wherein step (e) comprises incubation of the polypeptide and connector compound together at 30° C. and at pH 8 in aqueous buffer comprising acetonitrile.
4. A method according to claim 1 , said method comprising the further step of (f) cleaving one or more bonds of the polypeptide chain.
5. A method according to claim 4 wherein said cleavage step comprises contacting said polypeptide with a protease.
6. A method for identifying a complex according to claim 1 which is capable of binding to a ligand, the method comprising: (a) providing a complex according to claim 1 (b) contacting said complex with the ligand, and (c) selecting those complexes which bind said ligand.
7. A method for identifying a complex according to claim 1 , wherein the the polypeptide comprises a sequence SEQ ID No. 1-5, 9, 10, or 36-63.