Methods and systems for performing digital measurements
Methods, devices and systems for performing digital measurements are provided.
1. A system for using digital measurements to determine a concentration of a sample, the system comprising:
a sample holder containing a plurality of droplets having a volume distribution wherein the volume distribution is a continuous volume distribution;
a detector for detecting a detectable agent contained in at least one droplet of the plurality; and
a computer comprising a memory device with executable instructions stored thereon, the instructions, when executed by a processor, cause the processor to:
analyze a portion of the plurality of droplets to determine individual volumes of the droplets in the portion of the plurality and a number of droplets in the portion of the plurality that contain a detectable agent; and
use individual volumes of the droplets in the portion of the plurality and the number of droplets in the portion of the plurality that contain the detectable agent to determine the concentration of the sample.
2. The system of claim 1 , wherein the plurality of droplets is produced in an emulsion by combining immiscible fluids.
3. The system of claim 2 , wherein the immiscible fluids comprise water and oil.
4. The system of claim 1 , wherein the detectable agent is fluorescent.
5. The system of claim 4 , wherein the detectable agent is associated with a nucleic acid molecule, a peptide, a protein, or a combination thereof.
6. The system of claim 1 , wherein at least one of the droplets in the plurality comprises an amplified product from a polymerase chain reaction (PCR), rolling circle amplification (RCA), nucleic acid sequence based amplification (NASBA), loop-mediated amplification (LAMP), or a combination thereof.
7. The system of claim 1 , wherein the concentration of the detectable agent is determined over a dynamic range of at least three orders of magnitude.
8. The system of claim 1 , wherein the concentration of the detectable agent is determined over a dynamic range of at least six orders of magnitude.
9. The system of claim 1 , wherein the volumes in the volume distribution vary by more than a factor of 2.
10. The system of claim 1 , wherein the volumes in the volume distribution vary by more than a factor of 10 or by more than a factor of 100.