Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
View Patent ↗The present invention relates to isolated polypeptides having cellobiohydrolase activity, catalytic domains, and cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains, and cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, or cellulose binding domains.
1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having cellobiohydrolase activity has at least 95% sequence identity to amino acids 20 to 456 of SEQ ID NO: 2.
2. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity is encoded by a polynucleotide which is identical to the polynucleotide contained in plasmid pAJ227 which is contained in E. coli deposited with the Agricultural Research Service Patent Culture Collection (NRRL) under accession number B-50474 or which is identical to the polypeptide encoded by the polynucleotide contained in plasmid pAJ227 which is contained in E. coli deposited with the Agricultural Research Service Patent Culture Collection (NRRL) under accession number B-50474.
3. A recombinant isolated host cell comprising the nucleic acid construct of claim 1 .
4. A method of producing a polypeptide having cellobiohydrolase activity, said method comprising:
(a) cultivating the host cell of claim 3 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
5. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to amino acids 20 to 456 of SEQ ID NO: 2.
6. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 98% sequence identity to amino acids 20 to 456 of SEQ ID NO: 2.
7. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 99% sequence identity to amino acids 20 to 456 of SEQ ID NO: 2.
8. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises amino acids 20 to 456 of SEQ ID NO: 2.
9. The method of claim 4 , wherein the polypeptide having cellobiohydrolase activity comprises amino acids 20 to 456 of SEQ ID NO: 2.
10. The method of claim 4 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to amino acids 20 to 456 of SEQ ID NO: 2.
11. A recombinant isolated host cell transformed with a nucleic acid constrict or expression vector comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polypeptide having cellobiohydrolase activity has at least 95% sequence identity to amino acids 20 to 456 of SEQ ID NO: 2 and wherein the polynucleotide is operably linked to a heterologous promoter.
12. The recombinant isolated host cell of claim 11 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to amino acids 20 to 456 of SEQ ID NO: 2.
13. The recombinant isolated host cell of claim 11 , wherein the polypeptide having cellobiohydrolase activity has at least 99% sequence identity to amino acids 20 to 456 of SEQ ID NO: 2.
14. The recombinant isolated host cell of claim 11 , wherein the polypeptide having cellobiohydrolase activity comprises amino acids 20 to 456 of SEQ ID NO: 2.