SIRTUIN MODULATORS AS INHIBITORS OF CYTOMEGALOVIRUS
Agents and methods of inhibiting or improving the growth of viruses are provided.
1 . A method of inhibiting virus production comprising contacting a virus-infected cell with one or more inhibitors of two or more sirtuins, wherein the sirtuins are selected from SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6, and SIRT7.
2 .- 10 . (canceled)
11 . A method of inhibiting virus production comprising contacting a virus-infected cell with a SIRT1 inhibitor and a SIRT2 inhibitor.
12 . The method of claim 11 , wherein the SIRT1 inhibitor and the SIRT2 inhibitor are a dual SIRT1/SIRT2 inhibitor.
13 . (canceled)
14 . The method of claim 12 , wherein the dual SIRT1/SIRT2 inhibitor is cambinol, salermide, tenovin-6, tenovin-1, or a combination thereof.
15 .- 20 . (canceled)
21 . The method of claim 1 , wherein the virus is HCMV, human papilloma virus, HSV1, FluA, adenovirus, influenza virus, hepatitis B virus, hepatitis C virus, or HIV.
22 . The method of claim 11 , wherein the virus is Adenoviridae; Hepadnaviridae; Herpesviridae; Flaviviridae; Orthomixoviridae; Papillomaviridae; Polyomaviridae; or Retroviridae.
23 . The method of claim 11 , further comprising contacting the cell with one or more of a SIRT3, SIRT4, SIRT5, SIRT6, and SIRT7 agonist.
24 . (canceled)
25 . The method of claim 1 , further comprising contacting the virus with a second agent.
26 . The method of claim 25 , wherein the second agent is an antiviral agent.
27 .- 28 . (canceled)
29 . The method of claim 1 , wherein the cell is a tumor cell.
30 . The method of claim 29 , wherein the tumor cell is infected with HCMV.
31 . The method of claim 1 , wherein the two or more sirtuins are inhibited by inhibiting gene expression of at least one of the two or more sirtuins.
32 .- 36 . (canceled)
37 . The method of claim 1 , wherein the contacting is in vitro.
38 . The method of claim 1 , wherein the contacting is in vivo.
39 .- 46 . (canceled)
47 . A method of increasing virus production comprising inhibiting sirtuin gene expression in a virus-infected cell.
48 .- 54 . (canceled)
55 . The method of claim 47 , wherein the sirtuin gene expression is inhibited by contacting the cell with a dominant negative sirtuin gene, coding sequence or protein.
56 . The method of claim 47 , wherein the sirtuin gene expression is inhibited in cells from a transgenic animal.
57 . The method of claim 47 , wherein the sirtuin gene expression is inhibited by mutational inactivation within cultured cells.