Coryneform bacterium transformant and process for producing phenol using the same
View Patent ↗Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.
1. A phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a Corynebacterium glutamicum as a host.
2. The transformant of claim 1 , wherein the gene which encodes an enzyme having tyrosine phenol-lyase activity is a gene derived from Pantoea agglomerans , a gene derived from Citrobacter braakii , a gene derived from Desulfitobacterium hafniense , a gene derived from Chloroflexus aurantiacus , a gene derived from Nostoc punctiforme , or a gene derived from Treponema denticola.
3. The transformant of claim 1 , wherein the gene which encodes an enzyme having tyrosine phenol-lyase activity is the DNA of the following (a) or (b),
(a) a DNA consisting of the base sequence of SEQ ID NO: 16, a DNA consisting of the base sequence of SEQ ID NO: 23, a DNA consisting of the base sequence of SEQ ID NO: 24, a DNA consisting of the base sequence of SEQ ID NO: 25, a DNA consisting of the base sequence of SEQ ID NO: 26, or a DNA consisting of the base sequence of SEQ ID NO: 27
(b) a DNA which hybridizes to a DNA consisting of a complementary base sequence of any of the DNAs of (a) under stringent conditions and which encodes a polypeptide having tyrosine phenol-lyase activity,
wherein the stringent conditions are conditions where hybridization occurs at a temperature 10° C. below the melting temperature (Tm) of a perfect hybrid.
4. The transformant of claim 1 , wherein the Corynebacterium glutamicum as the host is a Corynebacterium glutamicum in which a gene which encodes an enzyme having phenol 2-monooxygenase activity on the chromosome is disrupted or deleted.
5. The transformant of claim 1 , wherein the Corynebacterium glutamicum as the host is Corynebacterium glutamicum R (FERM BP-18976), ATCC13032, or ATCC13869.
6. The transformant of claim 1 , wherein the Corynebacterium glutamicum as the host is a strain of Corynebacterium glutamicum R (FERM BP-18976), ATCC13032, or ATCC13869 in which a gene which encodes an enzyme having phenol 2-monooxygenase activity on the chromosome is disrupted or deleted.
7. A Corynebacterium glutamicum transformant PHE31 (Accession Number: NITE BP-999).
8. A process for producing phenol, which comprises a step of allowing the transformant of claim 1 to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.
9. The process of claim 8 , wherein the transformant does not proliferate in the reaction step.
10. The process of claim 8 , wherein the oxidation-reduction potential of the reaction mixture under reducing conditions is −200 mV to −500 mV.