Compositions comprising IL-7 receptor ligands
View Patent ↗Disclosed herein are novel proteins that have reduced binding to the interleukin-7 receptor, compositions containing such proteins, and methods of using the same.
1. An isolated IL-7 mutant protein, derived from a wild type IL-7 protein having the amino acid sequence as set forth in SEQ ID NO:7, having an amino acid substitution at site T97, wherein the IL-7 mutant protein has reduced binding to an IL-7 receptor compared to binding of the wild type IL-7 protein to the IL-7 receptor.
2. The IL-7 mutant protein of claim 1 , wherein the binding of the IL-7 mutant protein to the IL-7 receptor causes an attenuated signal by the receptor.
3. The IL-7 mutant protein of claim 1 , wherein the T97 is substituted with valine.
4. A composition comprising the IL-7 mutant protein of claim 1 .
5. The composition of claim 4 , wherein the T97 of the IL-7 mutant protein is substituted with valine.
6. The composition of claim 4 , further comprising a pharmaceutically acceptable carrier.
7. An isolated nucleic acid encoding an IL-7 mutant protein derived from a wild type of IL-7 protein having the amino acid sequence as set forth in SEQ ID NO:7, having an amino acid substitution at site T97, wherein the IL-7 mutant protein has reduced binding to an IL-7 receptor compared to binding of the wild type IL-7 protein to the IL-7 receptor.
8. The isolated nucleic acid of claim 7 , comprising the nucleotide sequence of SEQ ID NO:3.
9. A vector comprising the nucleic acid of claim 7 or claim 8 .
10. An isolated host cell comprising the nucleic acid of claim 7 or claim 8 .