Device for nucleic acid analysis and nucleic acid analyzer
View Patent ↗Sample nucleic acids are prevented from removing from a sample-immobilizing layer during a sequencing reaction. A reaction device for nucleic acid analysis is provided that enables high throughput analysis by increasing the number of nucleic acid samples that can be analyzed. The reaction device for nucleic acid analysis comprises a substrate, a sample-immobilizing layer on the substrate, nucleic acid samples or carriers having nucleic acid samples on their surfaces, which are immobilized on the sample-immobilizing layer, and a blocking layer that covers areas other than areas where the nucleic acid samples or the carriers are bound to the sample-immobilizing layer, wherein the immobilizing layer is formed of an inorganic oxide.
1. A reaction device for nucleic acid analysis, comprising:
a substrate,
a sample-immobilizing layer on the substrate,
a nucleic acid sample or a carrier having a nucleic acid sample on its surface, which is immobilized on the sample-immobilizing layer through phosphate group or phosphoester bond of the nucleic acid, and
a blocking layer that covers an area other than an area where the nucleic acid sample or the carrier is bound to the sample-immobilizing layer, wherein the sample-immobilizing layer is formed of an inorganic oxide, the blocking layer is selected from the group consisting of a carboxylic compound, a sulfuric acid compound, a salt thereof, and a mixture of at least 2 types of these compounds, the compound used for the formation of the blocking layer has a hydrophobic site, the hydrophobic site is long chain alkyl group, a carboxylic acid group and/or sulfate group within the compound binds to the inorganic oxide, and the inorganic oxide is selected from the group consisting of titania, zirconia, alumina, zeolite, vanadium pentoxide, silica, sapphire, tungsten oxide, tantalum pentoxide, and a mixture of at least 2 types thereof.
2. The reaction device for nucleic acid analysis according to claim 1 , comprising:
a first substrate that comprises the sample-immobilizing layer, a nucleic acid sample or a carrier having a nucleic acid sample on its surface, which is immobilized on the sample-immobilizing layer through phosphate group or phosphoester bond of the nucleic acid, and
a blocking layer that covers an area other than an area where the nucleic acid sample or the carrier is bound to the sample-immobilizing layer, wherein the sample-immobilizing layer is formed of an inorganic oxide;
a second substrate; and
a spacer having a hollow part for formation of a flow channel, wherein
the first substrate and the second substrate are adhered to each other via the spacer to form the flow channel between the substrates,
within the flow channel, the sample-immobilizing layer, the nucleic acid sample or the carrier having the nucleic acid sample on its surface, which is immobilized on the immobilizing layer, and the blocking layer that covers an area other than an area where the nucleic acid sample or the carrier is bound to the sample-immobilizing layer are present, and
a solution inlet port and a solution outlet port that are in communication with the flow channel are provided.
3. The reaction device for nucleic acid analysis according to claim 2 , wherein the spacer has a thickness of 1 mm or less.