Grafted dinuclear metal complexes, and use thereof as cellular microparticle sensors
Novel grafted dinuclear metal complexes having formula (I) or of formula (II) wherein M represents a metal ion, and also the use thereof as sensors in a method for detecting and/or characterising cellular microparticles.
1. A compound having formula (I) or formula (II):
wherein
M +i is a metal ion, and i is 1, 2 or 3;
L is an exchangeable ligand;
X is a —(CH 2 ) m —NH-A group, or a —CH 2 —NHC(O)—R—NH-A group wherein R is a C 2 -C 10 alkyl group, preferably a C 6 -C 10 alkyl group, either substituted or unsubstituted, linear or branched, and A is H or a fluorophore group;
m=1 to 12;
n=1, 2 or 3; and
Y is —(CH 2 ) p —NH 2 wherein p=0 to 12.
2. A compound having formula (I) or formula (II) according to claim 1 , characterised in that M is selected from Zn, Cu, Mn, Co, Ni and Fe.
3. A compound according to claim 1 having formula (I) or (II) wherein:
M is Zn or Cu;
X is —(CH 2 ) m —NH-A, where m=1, A is H, n=1 and p=0; or
X is —(CH 2 ) m —NH-A, where m=1, A is H, n=2 and p=0; or
X is —CH 2 —NHC(O)—R—NH-A, A is H, n=1 and p=0.
4. A compound having formula (I) or formula (II) according to claim 1 , wherein X is —(CH 2 ) m —NH-A, —CH 2 —NHC(O)—R—NH-A wherein R is a C 2 -C 10 alkyl group, preferably a C 6 -C 10 alkyl group, either substituted or unsubstituted, linear or branched, and A is a fluorophore group.
5. A compound having formula (III)
wherein
Z is NH 2 or a —(CH 2 ) m —NH-A group, or a —CH 2 —NHC(O)—R—NH-A group wherein R is a C 2 -C 10 alkyl group, preferably a C 6 -C 10 alkyl group, either substituted or unsubstituted, linear or branched, and A is H or a fluorophore group, provided that when m=2, A is not H;
m=1 to 12;
n=1, 2 or 3; and
Y is (CH 2 ) p —NH 2 wherein p=0 to 12.
6. A compound having formula (III) according to claim 5 , wherein
z is —(CH 2 ) m —NH-A, where m=1, A is H, n=1 and p=0; or
z is —(CH 2 ) m —NH-A, where m=1, A is H, n=2 and p=0; or
z is —CH 2 —NHC(O)—R—NH-A, A is H, n=1and p=0.