Methods and compositions for the diagnosis, classification, and treatment of cancer
Some embodiments of the present technology relate to methods and compositions for the diagnosis and treatment of cancer. Some embodiments include methods and compositions for the diagnosis and treatment of castration-resistant prostate cancer. Some embodiments include methods and compositions for the diagnosis and treatment of pancreatic cancer.
1. A method for increasing the sensitivity of a neoplastic cell to a chemotherapeutic agent comprising:
reducing the expression level of a nucleic acid encoding c-Myb or the expression level of c-Myb protein in the cell or reducing activity of c-Myb protein by contacting the cell with a sufficient amount of an isolated nucleic acid to increase the sensitivity of said cell to said chemotherapeutic agent, wherein said isolated nucleic acid comprises at least 16 consecutive nucleotides having a sequence that is at least 90% identical to a sequence encoding c-Myb or complement thereof, wherein said isolated nucleic acid is selected from a small hairpin RNA (shRNA), a small interfering RNA (siRNA), a micro RNA (miRNA), an antisense polynucleotide, and a ribozyme, wherein the cell is selected from the group consisting of a prostate cancer cell, a castration-resistant prostate cancer cell, and an androgen-dependent prostate cancer cell; and
contacting the cell with an effective amount of the chemotherapeutic agent, wherein the effective amount is sufficient to inhibit the growth of the cell, wherein the effective amount is reduced compared to the effective amount for a cell wherein the level of a nucleic acid encoding c-Myb or a fragment thereof or the level of c-Myb protein or a fragment thereof or the activity of c-Myb protein is not reduced.
2. The method of claim 1 , wherein the isolated nucleic acid comprises a sequence selected from the group consisting of a sequence encoding antisense c-Myb or a fragment thereof, an antisense nucleic acid complementary to a sequence encoding c-Myb or a fragment thereof, and a sequence comprising SEQ ID NO:06.
3. The method of claim 1 , wherein the chemotherapeutic agent is selected from the group consisting of docetaxel, and paclitaxel.
4. A method for increasing the sensitivity of a neoplastic cell to a chemotherapeutic agent comprising reducing the expression level of a nucleic acid encoding c-Myb or the expression level of c-Myb protein in the cell or reducing activity of c-Myb protein by contacting the cell with a sufficient amount of an isolated nucleic acid to increase the sensitivity of said cell to said chemotherapeutic agent, wherein said isolated nucleic acid comprises SEQ ID NO:06.
5. The method of claim 4 , wherein the neoplastic cell is selected from the group consisting of a prostate cancer cell, a castration-resistant prostate cancer cell, and an androgen-dependent prostate cancer cell.
6. The method of claim 4 , further comprising contacting the cell with a chemotherapeutic agent.
7. The method of claim 6 , further comprising contacting the cell with an effective amount of the chemotherapeutic agent, wherein the effective amount is sufficient to inhibit the growth of the cell, wherein the effective amount is reduced compared to a cell wherein the level of a nucleic acid encoding c-Myb or a fragment thereof or the level of c-Myb protein or a fragment thereof or the activity of c-Myb protein is not reduced.
8. The method of claim 4 , wherein the chemotherapeutic agent is selected from the group consisting of docetaxel, and paclitaxel.
9. The method of claim 1 , wherein the isolated nucleic acid is selected from the group consisting of an siRNA and an shRNA.
10. The method of claim 1 , wherein the neoplastic cell is a prostate cancer cell.
11. The method of claim 1 , wherein the neoplastic cell is a castration-resistant prostate cancer cell.
12. The method of claim 1 , wherein the neoplastic cell is an androgen-dependent prostate cancer cell.
13. The method of claim 1 , wherein the chemotherapeutic agent is docetaxel.
14. The method of claim 1 , wherein the chemotherapeutic agent is paclitaxel.
15. The method of claim 1 , wherein the isolated nucleic acid comprises SEQ ID:06.
16. The method of claim 4 , wherein the expression level of a nucleic acid encoding c-Myb is reduced.
17. The method of claim 4 , wherein the expression level of c-Myb protein in the cell is reduced.
18. The method of claim 4 , wherein the activity of c-Myb protein is reduced.
19. The method of claim 4 , wherein the neoplastic cell is a prostate cancer cell.
20. The method of claim 4 , wherein the neoplastic cell is a castration-resistant prostate cancer cell.
21. The method of claim 4 , wherein the neoplastic cell is an androgen-dependent prostate cancer cell.
22. The method of claim 4 , wherein the chemotherapeutic agent is docetaxel.
23. The method of claim 4 , wherein the chemotherapeutic agent is paclitaxel.
24. The method of claim 6 , wherein the chemotherapeutic agent is docetaxel.
25. The method of claim 6 , wherein the chemotherapeutic agent is paclitaxel.