Proteases for degrading gluten
Gluten-degrading proteases derived from insects, including flour beetles, are isolated, and the purified, and recombinant forms can be used to make gluten-containing food safe for patients suffering from gluten intolerance.
1. A method for treating gluten intolerance in a patient in need of such treatment, wherein said treatment reduces exposure of said patient to immunogenic gluten peptides, said method comprising:
orally administering to said patient a therapeutically effective dose of an isolated, purified or recombinant form of one or more proteases selected from an insect protease comprising a sequence set forth in any one of SEQ ID NO:1, 2, 3, 4, 10, 11, 12, or 13 contemporaneously with the ingestion of a food that may contain gluten.
2. The method of claim 1 , further comprising orally administering to said patient a therapeutically effective dose of one or more non-insect proteases.
3. The method of claim 2 , wherein said non-insect protease is one or more of Hordeum vulgare endoprotease (Genbank accession U19384); X-Pro dipeptidase from Aspergillus oryzae (GenBank ID# BD191984); carboxypeptidase from Aspergillus saitoi (GenBank ID# D25288); Flavobacterium meningosepticum PEP (Genbank ID # D10980); Sphingomonas capsulate PEP (Genbank ID# AB010298); Penicillium citrinum PEP (Genbank ID# D25535); Lactobacillus helveticus PEP (Genbank ID# 321529); and Myxococcus xanthus PEP (Genbank ID# AF127082).
4. The method of claim 1 , wherein said upon ingestion with a gluten containing foodstuff said protease degrades the gluten to fragments shorter than 8 amino acids.
5. A method for treating gluten intolerance in a patient in need of such treatment, the method comprising:
orally administering to said patient a pharmaceutical formulation comprising an effective dose of an isolated, purified or recombinant form of an insect protease comprising the amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:11 in a unit dose pharmaceutical formulation comprising a pharmaceutically acceptable excipient, contemporaneously with the ingestion of a food that may contain gluten.
6. The method of claim 5 , further comprising orally administering to said patient a therapeutically effective dose of one or more non-insect proteases.
7. The method of claim 6 , wherein said non-insect protease is one or more of Hordeum vulgare endoprotease (Genbank accession U19384); X-Pro dipeptidase from Aspergillus oryzae (GenBank ID# BD191984); carboxypeptidase from Aspergillus saitoi (GenBank ID# D25288); Flavobacterium meningosepticum PEP (Genbank ID # D10980); Sphingomonas capsulata PEP (Genbank ID# AB010298); Penicillium citrinum PEP (Genbank ID# D25535); Lactobacillus helveticus PEP (Genbank ID# 321529); and Myxococcus xanthus PEP (Genbank ID# AF127082).
8. A method for treating gluten intolerance in a patient in need of such treatment, the method comprising:
orally administering to said patient a pharmaceutical formulation comprising:
an isolated, purified or recombinant form of a protease comprising the amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:11 in a unit dose of 0.01 mg to 500 mg /kg body weight, which upon oral administration by the patient is effective to cleave an ingested gluten oligopeptide to fragments shorter than 8 amino acids;
and a pharmaceutically acceptable excipient.
9. The method of claim 8 , further comprising orally administering to said patient a therapeutically effective dose of one or more non-insect proteases.
10. The method of claim 9 , wherein said non-insect protease is one or more of Hordeum vulgare endoprotease (Genbank accession U19384); X-Pro dipeptidase from Aspergillus oryzae (GenBank ID# BD191984); carboxypeptidase from Aspergillus saitoi (GenBank ID# D25288); Flavobacterium meningosepticum PEP (Genbank ID # D10980); Sphingomonas capsulate PEP (Genbank ID# AB010298); Penicillium citrinum PEP (Genbank ID# D25535); Lactobacillus helveticus PEP (Genbank ID# 321529); and Myxococcus xanthus PEP (Genbank ID# AF127082).