Cysteine hydrazide nicotinamide for glycomics and glycoproteomics uses
A cysteine hydrazide nicotinamide (Cyhn) reagent designed for the enrichment of bacterial glycoproteins is provided. Methods for purification of free oligosaccharides and their analysis are also provided.
1. A compound having a structure of Formula (II):
or a salt thereof, wherein:
n is an integer from 1-10;
R is a nitrogen-containing ring capable of forming a salt;
wherein the nitrogen-containing ring is a ring selected from the group consisting of pyridyl, pyridazinyl, pyrazinyl, quinoxalyl, indolizinyl, quinazolinyl, purinyl, quinolinyl, pyrimidinyl, pyrrolyl, pyrazolyl, tetrazolyl, imidazolyl, triazolyl, indolyl, piperidinyl, piperazinyl, and pyrrolidinyl; and
R 1 is H or saccharide.
2. The compound according to claim 1 , wherein R is pyridyl.
3. The compound according to claim 1 , wherein n=1.