Method for making pluripotent stem cells
The present application discloses a method for inducing cells to gain characteristics of naïve stem cell state comprising culturing the cells in the presence of a MUC1* activator.
1. An article comprising a stem cell proliferation surface without feeder layer, to which is bound an agent that binds to a cell surface molecule that is present on stem cells or progenitor cells, wherein the agent is monoclonal antibody that binds to PSMGFR, wherein the monoclonal antibody has the following Kappa Chain Variable Region CDR sequences:
(SEQ ID NO: 20)
CDR1: RSSQTIVHSNGNTYLE;
(SEQ ID NO: 21)
CDR2: KVSNRFS;
and
(SEQ ID NO: 22)
CDR3: FQGSHVPFT.
2. The article according to claim 1 , wherein the surface comprises from at least about 0.5% Nitrogen (N), at least about a sum of Oxygen (O) and Nitrogen (N) of greater than or equal to 17.2%, and a contact angle of at least about 13.9 degrees.
3. The article according to claim 1 , wherein the surface is Vita or Vita-like surface.
4. The article according to claim 1 , wherein the cell surface molecule is MUC1.
5. The article according to claim 4 , wherein the cell surface molecule is MUC1*.
6. The article according to claim 5 , wherein the cell surface molecule is consists essentially of the PSMGFR sequence.