Polynucleotides encoding IL-31 monoclonal antibodies
The present invention relates to methods of treating pruritic diseases, including but not limited to Contact dermatitis, Atopic Dermatitis, Drug induced delayed type cutaneous allergic reactions, Toxic epidermal necrolysis, Cutaneous T cell Lymphoma, Bullous pemphigoid, Alopecia wereata, Vitiligo, Acne Rosacea, Prurigo nodularis, Scleroderma, Herpes simplex virus, or combination thereof by administering IL-31 monoclonal antibodies. The invention further provides the hybridomas that generate the monoclonal antibodies.
1. An isolated polynucleotide encoding a monoclonal antibody or antigen-binding fragment thereof that specifically binds to a polypeptide consisting of amino acid residues 27-164 of SEQ ID NO:2, wherein the monoclonal antibody or antigen-binding fragment thereof binds to the same epitope as the antibody produced by the hybridoma having ATCC Patent Deposit Designation PTA-6815 for binding to the polypeptide.
2. The polynucleotide of claim 1 , wherein the monoclonal antibody is the antibody produced by the hybridoma having ATCC Patent Deposit Designation PTA-6815.
3. The polynucleotide of claim 2 , wherein the monoclonal antibody is humanized.
4. The polynucleotide of claim 3 , wherein the humanized monoclonal antibody comprises a human immunoglobulin constant region.
5. The polynucleotide of claim 3 , wherein the isotype of the humanized monoclonal antibody is IgG.
6. The polynucleotide of claim 1 , wherein the antigen-binding fragment is a Fab, Fab′, F(ab′) 2 , or single chain Fv.
7. An isolated vector comprising the polynucleotide of claim 1 .
8. An isolated host cell comprising the vector of claim 7 .
9. A method of producing a monoclonal antibody or antigen-binding fragment thereof that specifically binds to a polypeptide consisting of amino acid residues 27-164 of SEQ ID NO:2 comprising:
a) culturing the host cell of claim 8 under conditions wherein the monoclonal antibody or antigen-binding fragment is expressed; and
b) recovering the monoclonal antibody or antigen-binding fragment.