Methods, devices, kits and compositions for detecting roundworm
Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.
1. A device for detecting the presence or absence of roundworm antigens from a sample; the device comprising a solid support, wherein the solid support has immobilized thereon one or more antibodies that are capable of specifically binding to a polypeptide that has an amino acid sequence that corresponds to SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO: 11, or Copro6728.
2. The device of claim 1 wherein the one or more antibodies are obtained by immunization with the polypeptide that has an amino acid sequence that corresponds to SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO: 11, or Copro6728.
3. The device of claim 1 wherein the sample is a fecal sample.
4. The device of claim 3 wherein the one or more antibodies do not specifically bind any coproantigen derived from the group consisting of hookworm, whipworm, and heartworm.
5. The device of claim 1 wherein one or more of the one or more antibodies are labeled.
6. The device of claim 1 wherein the device is an enzyme-linked immunosorbent assay device.
7. The device of claim 6 wherein the enzyme-linked immunosorbent assay device is a lateral flow immunoassay device.
8. The device of claim 1 wherein the sample is from a canine or a feline.
9. The device of claim 1 wherein the device further includes one or more reagents for the detection of one or more of the group consisting of: one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi, and one or more bacteria.
10. A kit for detection of one or more roundworm antigens in a mammalian sample, the kit comprising the device of claim 1 , and one or more reagents sufficient for the detection of the one or more antigens.
11. The kit of claim 10 wherein the one or more reagents are selected from the group consisting of one or more indicator reagents, one or more antibody labeling compounds, one or more antibodies, one or more antigen capture reagents, one or more inhibitors, and one or more wash reagents.