IP Library › Patent Application 14048548
Patent Application
App. No. 14/048,548

DETECTION KIT CONTAINING A NOVEL RECOMBINANT 15-kDA POLYPEPTIDE USEFUL FOR DETECTING HUMAN INFECTION WITH BARTONELLA HENSELAE

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Patent No.
US None
App. No.
14/048,548
Abstract

Disclosed are the cloning and expression of a novel antigen of Bartonella henselae . The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.

Claims (14)

1 . A composition for detection of the presence of anti-15-kDa IgG or IgM antibodies in a human suspected of Bartonella henselae infection, comprising:

a) an isolated recombinant 15-kDa polypeptide, said isolated recombinant 15-kDa polypeptide having an amino acid sequence of SEQ ID NO: 2; and

b) a support, said support having said isolated recombinant 15-kDa polypeptide immobilized thereon,

wherein said composition detects the presence of anti-15-kDa IgG or IgM antibodies in a human suspected of Bartonella henselae infection.

2 . The composition of claim 1 , wherein said support is selected from the group consisting of polyethylene, polypropylene and glass.

3 . The composition of claim 2 , wherein said support is polypropylene.

4 . The composition of claim 1 , wherein said support is a microtiter well.

5 . The composition of claim 1 , further comprising:

c) packaging material.

6 . The composition of claim 5 , wherein said packaging material comprises an instruction for detecting the presence of an antibody against Bartonella henselae.

7 . The composition of claim 1 , further comprising:

d) a vector and a promoter.

8 . The composition of claim 7 , wherein said vector is selected from the group consisting of pET, pENTR and pCR 8/GW/TOPO and said promoter is selected from the group consisting of lac promoter, trp promoter and tac promoter.

9 . The composition of claim 8 , wherein said vector is pET and said promoter is lac promoter.

Assignments (1)
SECURITY INTEREST Recorded Apr 27, 2018
From: MEDICAL DIAGNOSTIC LABORATORIES, L.L.C.
To: TD BANK, N.A.
Reel/Frame 046031/0381 →