Exosomes and diagnostic biomarkers
View Patent ↗The invention provides methods for the detection of M. tuberculosis proteins in or on exosomes derived from infected individuals. The methods can use a proteomic approach including mass spectroscopy, data mining and multiplex reaction monitoring to quickly examine a large amount of M. tuberculosis proteins to determine the best biomarkers for use in diagnostic tests to identify active TB patients.
1. A protein signature diagnostic of an active M. tuberculosis infection wherein the protein signature of the active M. tuberculosis infection comprises proteins present on or in exosomes, the proteins comprising the combination of Rv0129c, Rv0440, Rv0934, Rv1837c, Rv1860, Rv1886c, Rv1980c, Rv2031c, Rv2376c, Rv3248, Rv3418c, Rv3441c, Rv3804c, and Rv3841, and acetonitrile or formic acid.
2. The protein signature of claim 1 wherein the protein signature is in contact with acetonitrile.
3. The protein signature of claim 1 wherein the protein signature is in contact with formic acid.
4. The protein signature of claim 1 comprising about 3% acetonitrile.
5. The protein signature of claim 1 comprising about 0.1% formic acid.
6. The protein signature of claim 3 comprising about 3% acetonitrile and 0.1% formic acid.
7. The protein signature of claim 1 further comprising Rv0350, Rv1469, Rv2244 and Rv2626c.
8. The protein signature of claim 1 further comprising Rv2626c.
9. A protein signature diagnostic of an active M. tuberculosis infection wherein the protein signature of the active M. tuberculosis infection comprises proteins present on or in exosomes, the proteins comprising the combination of Rv0129c, Rv0440, Rv0934, Rv1837c, Rv1860, Rv1886c, Rv1980c, Rv2031c, Rv2376c, Rv3248, Rv3418c, Rv3441c, Rv3804c, and Rv3841, and sodium dodecyl sulfate.
10. The protein signature of claim 8 further comprising Rv0350, Rv1469, Rv2244 and Rv2626c.
11. The protein signature of claim 8 further comprising Rv2626c.