Methods and compositions for the treatment of cancer or other diseases
The present invention relates to methods and compositions for the treatment of diseases, including cancer, infectious diseases and autoimmune diseases. The present invention also relates to methods and compositions for improving immune function. More particularly, the present invention relates to multifunctional molecules that are capable of being delivered to cells of interest for the treatment of diseases and for the improvement in immune function.
1. A molecule comprising a first oligonucleotide moiety covalently bound to a first oligonucleotide through a first spacer, wherein:
(i) said first oligonucleotide is hybridized to a second complementary oligonucleotide, wherein said second complementary oligonucleotide is covalently bound to a dsRNA through a second spacer, wherein said second complementary oligonucleotide and the complementary portion of said first oligonucleotide comprise 2′-fluoro, 2′-O-methyl or locked nucleic acid; and
(iii) said first spacer and said second spacer consist of one or more C3 spacers, wherein said dsRNA is an siRNA and said first oligonucleotide moiety comprises an aptamer.
2. The molecule of claim 1 , wherein the first oligonucleotide moiety is capable of delivering the molecule to a cell of interest.
3. The molecule of claim 2 , wherein the dsRNA is capable of treating a disease.
4. The molecule of claim 3 , wherein the disease is selected from the group consisting of cancer, an infectious disease, an autoimmune disease, a disease due to excessive angiogenesis and/or a disease benefitting from increased angiogenesis.