Type II restriction-modification system methylation subunit of
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.
1. A method of modulating or altering recombination, the method comprising:
providing an isolated or purified polypeptide having at least 90% sequence identity to SEQ ID No. 2976 to a nucleotide sequence in which a genetic recombination event is desired.
2. The method according to claim 1 , wherein the polypeptide has activity at or below about pH 8.
3. The method according to claim 1 , wherein the polypeptide has activity at a temperature at or above about 50 degrees Celsius.
4. The method according to claim 1 , wherein the modulation or alteration of recombination occurs at or below about pH 8.
5. The method according to claim 1 , wherein the modulation or alteration of recombination occurs at a temperature at or above about 50 degrees Celsius.
6. The method according to claim 1 , wherein the purified or isolated polypeptide is glycosylated, pegylated, or otherwise post-translationally modified.
7. The method according to claim 1 , wherein the polypeptide has as a Type II restriction-modification system methylation subunit.