Multimeric oxidoreductases
Described herein are multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate. The complexes comprise a dehydrogenase subunit and a cytochrome C subunit. Also described are polynucleotides coding for the multimeric complexes and methods of use thereof.
1. A method for producing a desired product comprising,
contacting an altered bacterial host cell with a polyol substrate under suitable culture conditions, wherein the altered bacterial host cell is obtained by the transformation of a bacterial host cell with a polynucleotide coding for a multimeric oxidoreductase complex selected from SEQ ID NO: 5 and SEQ ID NO: 23 and wherein the polynucleotide encoding the multimeric oxidoreductase complex is expressed in the altered bacterial host cell and
producing a desired product from an enzymatic conversion of the polyol substrate by the multimeric oxidoreductase complex to said desired product.
2. The method according to claim 1 , wherein the polyol substrate is glucose, gluconate, arabinose, arabitol, xylose, xylulose, xylitol, sucrose, sorbose, fructose, mannose, mannitol, meso-erythritol, L-erythrulose, idonate, cellobiose, lactose, idose, galactose, ribose, altose, maltose, erythrose, sorbitol, glycerol, 2-KDG, 5-keto-D-gluconic acid, 2-KLG, methanol, and erythorbic acid.
3. The method according to claim 1 , wherein the desired product is an ascorbic acid (AsA) intermediate.
4. The method according to claim 3 , wherein the AsA intermediate is 2-keto-D-gluconate or 2,5-diketo-D-gluconate.
5. The method according to claim 1 , wherein the desired product is an organic acid.
6. The method according to claim 1 , wherein the host cell is selected from an Enterobacteriaceae strain, optionally a Pantoea, Klebsiella, Erwinia or E. coli strain or a Pseudomonadacea strain.
7. The method according to claim 1 further comprising recovering the desired product.
8. The method according to claim 2 , wherein the polyol substrate is glucose, sorbitol or glycerol.
9. The method according to claim 1 , wherein the desired product is a sugar acid.
10. The method according to claim 1 , wherein said polynucleotide encoding the multimeric oxidoreductase complex further comprises SEQ ID NO: 41.