Recombinant cell surface capture proteins
Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and/or substituted CH3 domain are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and/or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).
1. A recombinant antigen-binding protein that binds a human IgG1-Fc domain, a human IgG2-Fc domain, or a human IgG4-Fc domain, wherein the antigen-binding protein comprises a heavy chain CDR-1 (HCDR-1) having the amino acid sequence of SEQ ID NO:27, an HCDR-2 having the amino acid sequence of SEQ ID NO:28, an HCDR-3 having the amino acid sequence of SEO ID NO:29, and a light chain variable region having the amino acid sequence of SEO ID NO: 16.
2. The recombinant antigen-binding protein of claim 1 , wherein the antigen-binding protein binds a polypeptide comprising an amino acid sequence of SEQ ID NO:26.
3. The recombinant antigen-binding protein of claim 2 , wherein the antigen-binding protein binds the polypeptide with a KD of less than about 40 nM as measured in a surface plasmon resonance assay.
4. The recombinant antigen-binding protein of claim 2 , wherein the antigen-binding protein comprises an HCVR having an amino acid sequence that is at least 95% identical to SEQ ID NO:15.
5. The recombinant antigen-binding protein of claim 4 , wherein the antigen-binding protein comprises an HCVR having the amino acid sequence of SEQ ID NO:15.
6. The recombinant antigen-binding protein of claim 4 , wherein the antigen-binding protein is an ScFv fusion protein comprising (a) a heavy chain variable domain comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:15, and (b) a membrane anchor domain comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:17 or SEQ ID NO:21.
7. The recombinant antigen-binding protein of claim 6 , wherein the antigen-binding protein is an ScFv fusion protein comprising a heavy chain variable domain that has an amino acid sequence identical to SEQ ID NO:15.
8. The recombinant antigen-binding protein of claim 7 , wherein the antigen-binding protein is an ScFv fusion protein comprising the amino acid sequence of SEQ ID NO:19.