Degradable Clostridial toxins
The specification discloses Clostridial toxins or Clostridial toxin chimeras comprising an inactivation cleavage site, polynucleotide molecules encoding such toxins or chimeras, compositions comprising such toxins or chimeras, and method of producing such toxins or chimeras.
1. A non-naturally occurring polynucleotide molecule comprising SEQ ID NO:536 encoding a non-naturally occurring Clostridial toxin comprising a mutation of an inactivation cleavage site.
2. A method of producing a non-naturally occurring botulinum neurotoxin comprising the steps of:
a. introducing into an Escherichia coli cell a polynucleotide molecule of claim 1 ; and
b. expressing the polynucleotide molecule, wherein expression from the polynucleotide molecule produces the encoded variant botulinum neurotoxin.