Fusion proteins useful for producing pinene
View Patent ↗The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.
1. A fusion protein comprising: (a) a pinene synthase (PS), or enzymatically active fragment thereof, and (b) a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment thereof, wherein the C-terminus of (b) is linked to the N-terminus of (a), and (a) and (b) are linked by a linker which is a peptide of between 3 and 9 amino acid residues; wherein the PS is a Pinus taeda, Picea abies , or Abies grandis PS; and the GPPS is a Picea abies or Abies grandis GPPS.
2. The fusion protein of claim 1 , wherein the linker is a peptide of between 5 and 7 amino acid residues.
3. The fusion protein of claim 2 , wherein the linker is a peptide of 6 amino acid residues.
4. The fusion protein of claim 3 , wherein the peptide of 6 amino acid residues has the amino acid sequence GSGGSG (SEQ ID NO:1).
5. The fusion protein of claim 1 , wherein the PS is a Picea abies or Abies grandis PS.
6. The fusion protein of claim 1 , wherein the GPPS is an Abies grandis GPPS.
7. The fusion protein of claim 1 , wherein the PS and GPPS are from a same species.
8. The fusion protein of claim 7 , wherein the species is Picea abies.
9. The fusion protein of claim 7 , wherein the species is Abies grandis.