Transpulmonary liposome for controlling drug arrival
An object of the present invention is to provide a liposome which is excellent in intrapulmonary delivery controllability of drugs or genes and is suited for pulmonary administration. By modifying the surface of a liposome using a terminal hydrophobized polyvinyl alcohol and/or chitosan, retention of drugs or genes encapsulated in the liposome on the surface of lung tissue and transfer of drugs or genes into lung tissue can be properly modulated, and thus in vivo behavior can be controlled.
1. A method for treating a disease of lung tissue, comprising the step of administering to a lung of a patient suffering from the disease of lung tissue and needing fast-transfer of a drug or a gene into the lung tissue a therapeutically effective amount of a liposome preparation for pulmonary administration in which a drug or a gene is encapsulated in a liposome having a liposome membrane surface modified with a chitosan, thereby transferring the drug or the gene to the inside of a lung tissue,
wherein the liposome contains the chitosan in a proportion of 5 to 250 parts by weight, per 100 parts by weight of a total amount of all constituent components of the liposomal membrane; and
wherein the chitosan has a molecular weight from 100,000 to 150,000 daltons and a degree of deacetylation of 80% to 100%.
2. The method for treating a disease of lung tissue according to claim 1 , wherein the liposome contains a phospholipid as a constituent component of a liposomal membrane.
3. The method for treating a disease of lung tissue according to claim 1 ,
wherein the liposome for pulmonary administration contains phosphatidylcholine, cholesterol and a dialkyl phosphate ester as constituent components of the liposomal membrane.
4. The method for treating a disease of lung tissue according to claim 1 , wherein the gene is at least one nucleic acid selected from the group consisting of siRNA, mRNA, rRNA, micro RNA, ribozymes, antisense oligonucleotide, decoy oligonucleotide, plasmid DNA, peptide nucleic acid, tri plex forming oligonucleotide, aptamer, and isolated DNA.
5. The method for treating a disease of lung tissue according to claim 1 , wherein the lung administrated the liposome is that of the patient needing fast-transfer of the gene into the lung tissue.