Anti-microbial peptides and methods of use thereof
The present disclosure provides antimicrobial peptides, and compositions comprising same. The present disclosure further provides methods of inhibiting microbial growth.
1. A composition comprising:
a) an antimicrobial peptide, wherein the peptide has a length of from 10 amino acids to 50 amino acids, and wherein the peptide comprises an amino acid sequence having at least 95% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence
(SEQ ID NO: 63)
YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR,
wherein the peptide contains at least one phosphorylated tyrosine residue, and
b) a surfactant.
2. The antimicrobial peptide of claim 1 , wherein the peptide comprises an amino acid sequence selected from:
(SEQ ID NO: 1)
AIGGGLSSVGGGSSTIK;
(SEQ ID NO: 2)
AIGGGLSSVGGGSSTIKY;
(SEQ ID NO: 3)
RAIGGGLSSVGGGSSTIK;
(SEQ ID NO: 4)
GGLSSVGGGSSTIK;
(SEQ ID NO: 5)
AIGGGLSSVGGGS;
(SEQ ID NO: 6)
RAIGGGLSSVGGGSSTIKY;
(SEQ ID NO: 7)
ygsglgvgggfssssgraiggglssvgggsstiky;
(SEQ ID NO: 8)
YGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYT;
and
(SEQ ID NO: 9)
GGLSSVGGGS.
3. The antimicrobial peptide of claim 1 , wherein at least two adjacent amino acids of the peptide are joined via a linkage other than an amide bond.
4. The composition of claim 1 , wherein the antimicrobial peptide is present in an amount of from 0.5 weight % to 20 weight %.
5. The composition of claim 1 , further comprising one or more agents selected from the group consisting of a pH adjusting agent, a dye, a fragrance, and a complexing agent.
6. The composition of claim 1 , wherein the composition is a solid, a liquid, or a gel.
7. A pharmaceutical formulation comprising:
a) the composition of claim 1 ; and
b) a pharmaceutically acceptable excipient.
8. The composition of claim 1 , wherein the surfactant is a nonionic surfactant.
9. The composition of claim 1 , wherein the surfactant is an amphoteric surfactant.
10. The composition of claim 1 , wherein the surfactant is an anionic surfactant.
11. The composition of claim 1 , wherein the surfactant is a cationic surfactant.
12. The composition of claim 1 , wherein the peptide comprises an amino acid sequence having at least 98% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63).
13. The composition of claim 1 , wherein the peptide comprises an amino acid sequence having at least 99% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63).
14. A method of inhibiting growth of a bacterium, the method comprising contacting the bacterium with a growth inhibiting amount of an antimicrobial peptide, wherein the peptide has a length of from 10 amino acids to 50 amino acids, and wherein the peptide comprises an amino acid sequence having at least 95% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63), wherein the peptide contains at least one phosphorylated tyrosine residue.
15. The method of claim 14 , wherein said contacting is carried out in vitro.
16. The method of claim 15 , wherein the bacterium is on the surface of a medical device.
17. The method of claim 15 , wherein the bacterium is on a hard surface in a healthcare facility, an educational facility, a detention facility, or a residence.
18. The method of claim 14 , wherein the bacterium is in a liquid.
19. The method of claim 14 , wherein said contacting is in vivo.
20. A method of treating a bacterial infection in an individual in need thereof, the method comprising administering to the individual an effective amount of an antimicrobial peptide, wherein the peptide has a length of from 10 amino acids to 50 amino acids, and wherein the peptide comprises an amino acid sequence having at least 95% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63), wherein the peptide contains at least one phosphorylated tyrosine residue.