Method for generating potent dendritic cells
View Patent ↗Highly potent dendritic cells are generated in vivo or ex vivo by exposing precursor cells to an effective dose of IL-32.
1. A method of inducing dendritic cell differentiation from a mammalian precursor cell, the method comprising:
contacting a population of human monocytes with an effective dose of IL-32, for a period of time sufficient to induce differentiation of the precursor cells into dendritic cells wherein the contacting is performed ex vivo; and
isolating CD1b positive dendritic cells from said population.
2. The method of claim 1 , wherein the IL-32 is a human IL-32 protein.
3. The method of claim 1 , wherein the contacting is performed in the presence of an antigen of interest.
4. The method of claim 1 , wherein the human monocytes are isolated from a biological sample of peripheral blood mononuclear cells.
5. The method of claim 1 , wherein the dendritic cells are infused into an individual in need of increased immune responsiveness.
6. The method of claim 5 , wherein the dendritic cell is autologous or allogeneic with respect to the individual.