Peptide for inducing regeneration of tissue and use thereof
(Objective) An objective of the present invention is to provide therapeutic agents that, in association with stimulation of PDGFRα-positive cells such as bone marrow mesenchymal stem cells, promote their mobilization into blood and accumulation in a damaged tissue, and induce tissue regeneration in a living body. (Means for solution) Multiple peptides were synthesized, and the migration-promoting activity of each peptide was evaluated. As a result, the present inventors successfully identified multiple peptides that have migration-promoting activity on a PDGFRα-positive bone marrow mesenchymal stem cell line (MSC-1). Further, the present inventors confirmed that the identified peptides also have migration-promoting activity on skin fibroblasts, which are PDGFRα-positive cells.
1. A method of stimulating migration of a cell, mobilizing a cell from bone marrow to peripheral blood, and/or regenerating a tissue, in the body of a subject, wherein said method comprises administering to the subject an effective amount of a substance of any one of (a) to (c) below;
(a) a peptide consisting of a portion of an HMGB1 protein of SEQ ID NO:1 and having cell migration-stimulating activity;
(b) a cell secreting said peptide of (a); and
(c) a vector into which a DNA encoding said peptide of (a) is inserted, wherein said peptide has an activity of stimulating migration of a cell and said peptide consists of all or part of the amino acid sequence of positions 1 to 195 of SEQ ID NO:1.
2. The method, according to claim 1 , wherein said peptide has an activity of stimulating migration of a cell and said peptide consists of any of the amino acid sequences below with, optionally, 50 or fewer amino acids added:
(1) the amino acid sequence of position 17 to position 25 in the amino acid sequence of SEQ ID NO:1;
(2) the amino acid sequence of position 45 to position 74 in the amino acid sequence of SEQ ID NO:1;
(3) the amino acid sequence of position 55 to position 84 in the amino acid sequence of SEQ ID NO:1;
(4) the amino acid sequence of position 85 to position 169 in the amino acid sequence of SEQ ID NO:1; and
(5) the amino acid sequence of position 89 to position 185 in the amino acid sequence of SEQ ID NO:1.
3. The method, according to claim 1 , wherein said peptide has an activity of stimulating migration of a cell and said peptide does not comprise amino acids 186 to 215 of SEQ ID NO:1.
4. The method, according to claim 2 , wherein said peptide has an activity of stimulating migration of a cell and said peptide does not comprise amino acids 186 to 215 of SEQ ID NO:1.