Immobilized transaminases and process for making and using immobilized transaminase

1. An immobilized transaminase comprising:

a recombinant transaminase that is SEQ ID NO: 110; and

a resin, wherein the recombinant transaminase is attached to the resin, wherein the resin comprises polymethacrylate with epoxide functional groups, polymethacrylate with amino epoxide functional groups, styrene/DVB copolymer or polymethacrylate with octadecyl functional groups and wherein the immobilized transaminase loses less than 10% activity per hour in a solvent system that comprises at least 90% of organic solvents.

2. An immobilized transaminase of claim 1 wherein the immobilized transaminase loses less than 10% activity per hour in a solvent system that comprises at least 95% of organic solvents.

3. A process for making (2R)-4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-amine comprising the steps of:

1) dissolving 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-one in an organic solvent;

2) contacting 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-one with an immobilized transaminase of claim 1 in presence of an amino group.

4. A process of making an immobilized transaminase comprising:

1) incubating a solution of transaminase with a resin and an enzyme solution to form an immobilized transaminase, wherein that transaminase is SEQ ID NO: 110 and the resin comprises polymethacrylate with epoxide functional groups, polymethacrylate with amino epoxide functional groups, styrene/DVB copolymer or polymethacrylate with octadecyl functional groups;

2) filtering and rinsing the immobilized transaminase; and

3) drying the immobilized transaminase,

wherein the immobilized transaminase loses less than 10% activity per hour in a solvent system that comprises at least 90% of organic solvents.