Ribozyme for identifying modification on RNA sequence and RNA cleavage method using same
The hammerhead ribozyme according to the present invention produces a hammerhead ribozyme—target RNA construct by base-pairing with an edited target RNA, and an editing recognition site of the hammerhead ribozyme cleaves the modification site by forming a base pair with the target RNA modification site. The cleavage of the modification site is expected to be applicable to the research and development of new drugs that can be used to prevent or treat diseases caused by the edited target RNA.
1. A method for the cleavage of a target RNA comprising:
reacting a member selected from the group consisting of a hammerhead ribozyme represented by the formula of SEQ ID NO. 1:
a hammerhead ribozyme represented by the formula of SEQ ID NO. 3:
and a hammerhead ribozyme represented by the formula of SEQ ID NO. 5:
with a member selected from the group consisting of a target RNA represented by formula:
5′-GGAUCGGUAUGUAGCAAUACGU-A-AUCCUAUUGAGCAUGCC-3′ (SEQ ID NO:2), a target RNA represented by formula:
5′-UUCUAGCCUU-C-AGGAGUCAGGGC-3′ (SEQ ID NO:4) and a target RNA represented by formula:
5′-GCAGACAUAUAUGAU-A-CAAUUUGAUCAG-3′ (SEQ ID NO:6), respectively, to give a hammerhead ribozyme-target RNA construct represented by the formula of SEQ ID NOs. 1 and 2[IIIb]:
a hammerhead ribozyme-target RNA construct represented by the formula of SEQ ID NOs. 3 and 4[IIIc]:
and
a hammerhead ribozyme-target RNA construct represented by the formula of SEQ ID NOs. 5 and 6 [IIId]:
wherein arrowhead symbol means a cleaving site.