Polypeptides having endoglucanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polypeptide, which:
(a) has at least 95% sequence identity to the sequence of amino acids 27-409 of SEQ ID NO: 2, or
(b) is a fragment of the sequence of amino acids 27-409 of SEQ ID NO: 2, wherein the polypeptide has endoglucanase activity.
2. The polypeptide of claim 1 , which has at least 97% sequence identity to the sequence of amino acids 27-409 of SEQ ID NO: 2.
3. The polypeptide of claim 1 , which is a variant of the sequence of amino acids 27-409 of SEQ ID NO: 2, comprising a substitution, deletion, and/or insertion of one or more amino acids, wherein the variant has at least 98% sequence identity to the sequence of amino acids 27-409 of SEQ ID NO: 2.
4. The polypeptide of claim 1 , comprising the sequence of amino acids 27-409 of SEQ ID NO: 2.
5. The polypeptide of claim 1 , which is encoded by a polynucleotide that hybridizes with the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof under high stringency conditions.
6. The polypeptide of claim 1 , which is a fragment of the sequence of amino acids 27-409 of SEQ ID NO: 2.
7. A composition comprising a polypeptide of claim 1 and one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
8. A process for degrading a cellulosic material, comprising treating the cellulosic material with a polypeptide of claim 1 , a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
9. A process for degrading a cellulosic material, comprising treating the cellulosic material with a polypeptide of claim 2 , a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
10. A process for degrading a cellulosic material, comprising treating the cellulosic material with a polypeptide of claim 4 , a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
11. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition in the presence of a polypeptide of claim 1 , a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity; and
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product.
12. The process of claim 11 , further comprising recovering the fermentation product.
13. A recombinant host cell comprising a polynucleotide encoding the polypeptide of claim 1 , wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide.
14. A method of producing a polypeptide having endoglucanase activity, comprising:
(a) cultivating the host cell of claim 13 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
15. An isolated polypeptide comprising a catalytic domain, wherein the catalytic domain has at least 95% sequence identity to amino acids 27 to 332 of SEQ ID NO: 2 and has endoglucanase activity.
16. The polypeptide of claim 15 , which further comprises a carbohydrate binding module.
17. A composition comprising a polypeptide of claim 15 and one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
18. A process for degrading a cellulosic material, comprising treating the cellulosic material with a polypeptide of claim 15 , a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
19. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition in the presence of a polypeptide of claim 15 , a cellobiohydrolase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity; and
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product.
20. The process of claim 19 , further comprising recovering the fermentation product.