IP Library Granted Patent US 9,499,836
Granted Patent B2
US 9,499,836 · App. 14/119,426 · Granted Nov 22, 2016

Fertilization and fruit size

Inventors: Brian C. W. Crawford (San Diego, CA); Martin F. Yanofsky (San Diego, CA)
Assignee: The Regents of the University of California
C12N15/8287C07K14/415C12N15/829C12N15/8249C12N15/8261
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Quick Facts
Patent No.
US 9,499,836
App. No.
14/119,426
Granted
Nov 22, 2016
Kind
B2
Abstract

Plants having modulated expression of a HAF or BEE polypeptide and which modulate aspects of plant fertility, such as parthenocarpy, fertilization efficiency, and/or fruit size, are described. Methods of modulating fertility characteristics in a plant by modulating the expression of a HAF or BEE polypeptide in the plant are also described.

Claims (14)

1. A plant comprising a heterologous expression cassette, the expression cassette comprising a reproductive tissue-specific or an epidermal-specific promoter operably linked to a polynucleotide, wherein the polynucleotide encodes a polypeptide that is at least 90% identical to SEQ ID NO:2, wherein the plant has increased expression of the polypeptide compared to expression of the polypeptide in a non-transgenic control plant and wherein the plant exhibits one or more of increased fertilization efficiency, increased fruit size and/or parthenocarpy as compared to a non-transgenic control plant and wherein the increased fertilization efficiency is increased number of fertilized seeds per carpel.

2. The plant of claim 1 , wherein the polynucleotide encodes a polypeptide that is at least 95% identical to SEQ ID NO:2.

3. The plant of claim 2 , wherein the polynucleotide encodes a polypeptide that comprises SEQ ID NO:2.

4. The plant of claim 1 , wherein the promoter is tissue an epidermal-specific promoter.

5. The plant of claim 1 , wherein the promoter is a reproductive tissue-specific promoter.

6. The plant of claim 1 , wherein the plant is a fruit-bearing crop plant.

7. A plant cell from the plant of claim 1 .

8. A seed, flower, leaf, or fruit from the plant of claim 1 , wherein the seed, flower, leaf or fruit comprise said expression cassette.

9. An expression cassette comprising a heterologous reproductive tissue-specific, or epidermal-specific promoter operably linked to a polynucleotide, wherein the polynucleotide encodes a polypeptide that is at least 90% identical to SEQ ID NO:2, wherein introduction of the expression cassette into a plant results in the plant having one Or more of increased fertilization efficiency, increased fruit size and/or parthenocarpy, and wherein the increased fertilization efficiency is increased number of fertilized seeds per carpel, as compared to a non-transgenic control plant.

10. The expression cassette of claim 9 , wherein the polynucleotide encodes a polypeptide that is at least 95% identical to SEQ ID NO:2.

11. The expression cassette of claim 9 , wherein the promoter is an epidermal-specific promoter.

12. The expression cassette of claim 9 , wherein the promoter is a reproductive tissue-specific promoter.

13. An expression vector comprising the expression cassette of claim 9 .

14. The expression cassette of claim 9 , wherein the polynucleotide encodes a polypeptide that comprises SEQ ID NO:2.

Assignments (2)
CONFIRMATORY LICENSE Recorded Jul 30, 2014
From: UNIVERSITY OF CALIFORNIA SAN DIEGO
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 033446/0553 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 8, 2014
From: CRAWFORD, BRIAN C.W.; YANOFSKY, MARTIN F.
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 033104/0419 →
Continuity (2)
Provisional Application 61491574 · May 31, 2011
Related Publication 20140245490A1 · Aug 28, 2014