Polypeptides having xylanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A process for degrading a cellulosic material or xylan-containing material, comprising: treating the cellulosic material or xylan-containing material with one or more enzymes comprising a polypeptide having xylanase activity selected from the group consisting of
(a) a polypeptide having at least 85% sequence identity to the sequence of amino acids 21 to 405 of SEQ ID NO: 6; and
(b) a fragment of the sequence of amino acids 21 to 405 of SEQ ID NO: 6 that has xylanase activity.
2. The process of claim 1 , wherein the one or more enzymes further comprise a cellobiohydrolase, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
3. The process of claim 2 , wherein the polypeptide has at least 85% sequence identity to the sequence of amino acids 21 to 405 of SEQ ID NO: 6.
4. The process of claim 2 , wherein the polypeptide has at least 90% sequence identity to the sequence of amino acids 21 to 405 of SEQ ID NO: 6.
5. The process of claim 2 , wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 21 to 405 of SEQ ID NO: 6.
6. The process of claim 2 , wherein the polypeptide has at least 97% sequence identity to the sequence of amino acids 21 to 405 of SEQ ID NO: 6.
7. The process of claim 2 , wherein the polypeptide comprises the sequence of amino acids 21 to 405 of SEQ ID NO: 6.
8. The process of claim 2 , wherein the polypeptide is a variant of the sequence of amino acids 21 to 405 of SEQ ID NO: 6 comprising a substitution, deletion, and/or insertion at one or more positions and wherein the variant has at least 94% sequence identity to the sequence of amino acids 21 to 405 of SEQ ID NO: 6.
9. The process of claim 2 , wherein the polypeptide is a fragment of SEQ ID NO: 6, wherein the fragment has xylanase activity.
10. The process of claim 2 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under very high stringency conditions with the complement of the sequence of nucleotides 124-1517 of SEQ ID NO: 5.
11. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material or xylan-containing material with one or more enzymes comprising a polypeptide having xylanase activity selected from the group consisting of (i) a polypeptide having at least 85% sequence identity to the sequence of amino acids 21 to 405 of SEQ ID NO: 6; and (ii) a fragment of the sequence of amino acids 21 to 405 of SEQ ID NO: 6 that has xylanase activity; and
(b) fermenting the saccharified cellulosic material or xylan-containing material with one or more fermenting microorganisms to produce the fermentation product.
12. The process of claim 11 , wherein the one or more enzymes further comprise a cellobiohydrolase, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
13. The process of claim 12 , wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 6.
14. The process of claim 12 , wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 6.
15. The process of claim 11 , further comprising recovering the fermentation product from the fermentation.
16. A process for degrading a cellulosic material or xylan-containing material, comprising: treating the cellulosic material or xylan-containing material with one or more enzymes comprising a polypeptide having xylanase activity which comprises a catalytic domain having at least 90% sequence identity to the sequence of amino acids 21 to 337 of SEQ ID NO: 6.
17. The process of claim 16 , wherein the one or more enzymes further comprise a cellobiohydrolase, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide.
18. The process of claim 16 , wherein the polypeptide further comprises a cellulose binding domain.
19. A recombinant host cell transformed with a nucleic acid construct or expression vector operably linked to one or more control sequences that direct the production of the polypeptide, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in an expression host and wherein the polypeptide is selected from the group consisting of
(a) a polypeptide having at least 85% sequence identity to the sequence of amino acids 21 to 405 of SEQ ID NO: 6; and
(b) a fragment of the sequence of amino acids 21 to 405 of SEQ ID NO: 6 that has xylanase activity.
20. A method of producing a polypeptide having xylanase activity, comprising cultivating the host cell of claim 19 under conditions conducive for production of the polypeptide.
21. The method of claim 20 , further comprising recovering the polypeptide.