Opsonized β-glucan preparations and methods
Described herein are compositions that include isolated opsonized soluble β-glucan, methods of making those compositions and methods of using those compositions.
1. A composition consisting of:
isolated opsonized soluble β-(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose β-glucan derived from Saccharomyces cerevisiae able to bind to neutrophils and monocytes.
2. The composition of claim 1 wherein the isolated opsonized soluble β-(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose β-glucan comprises an opsonin coupled to the β(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose.
3. The composition of claim 2 wherein the opsonin is covalently coupled to the β(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose.
4. The composition of claim 3 wherein the opsonin is covalently coupled to the β(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose through a conjugation linker.
5. The composition of claim 2 wherein the opsonin comprises C3, C3a, C3b, iC3b, C3bB, C3bBb, C3d, C3dg, C4, C5, C5a, C5b, C6, C7, C8, C9, or any combination of two or more thereof.
6. A composition consisting of:
isolated iC3b-opsonized soluble β(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose βgulcan derived from Saccharomyces cerevisiae able to bind to neutrophils and monocytes.
7. The composition of claim 6 wherein the iC3b is covalently coupled to the soluble β(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose βgulcan.
8. The composition of claim 7 wherein the iC3b is covalently coupled to the soluble β(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose βgulcan through a conjugation linker.
9. The composition of claim 1 wherein the opsonin is covalently coupled to a plurality of soluble β(1,6)-[poly-1,3)-D-glucopyranosyl]-poly-β(1,3)-D-glucopyranose βglucan molecules.