Biological oxygen demand sensors
Bioelectrochemical Systems (BES) for use as Biological Oxygen Demand (BOD) sensors, systems incorporating BES sensors for measuring BOD, and methods of using the sensors and systems for measuring BOD. The disclosed sensors are inexpensive to construct, long-lasting, have a fast response, and a large dynamic range. The invention includes biological oxygen demand (BOD) sensors which incorporate at least three working electrodes, at least one counter electrode, a reservoir for dilution fluid, and a sensor for measuring an electric current or a voltage which flows from the working electrodes to the counter electrode. The BOD sensors will typically also include at least one electrically active microbe disposed in proximity to the working electrode.
1. A system for measuring a biological oxygen demand of a fluid, comprising
a bioelectrochemical system comprising a housing for first and second electrodes and a culture of exoelectrogenic bacteria, wherein the housing limits aerobic metabolism in proximity to the first electrode and the second electrode and the electrodes are configured to operate under anaerobic conditions;
a buffer storage system;
an electric current or voltage sensor operably coupled to the bioelectrochemical system; and
control electronics operably connected to the current or voltage sensor and the buffer storage system, and capable of receiving a measurement from the current or voltage sensor and causing the buffer storage system to contact the sample with a buffer.
2. The system of claim 1 , wherein the culture of exoelectrogenic bacteria comprises a species selected from the genera Bacillus, Geobacter, Shewanella, Clostridia, Pseudomonas, Desulfovibrio, Desulfuromonas, Desulfobulbus, Rhodoferax , or Escherichia or combinations thereof.
3. A method for determining a biological oxygen demand (BOD) of a sample, comprising:
diluting the sample with a dilution fluid to obtain at least three different dilutions of the sample;
measuring the BOD of each of the at least three different dilutions using a bioelectrochemical system to obtain at least three BOD values; and
comparing the at least three BOD values to determine a BOD of the sample; and
determining a minimum dilution rate to enhance accuracy.
4. The method of claim 3 , wherein measuring the BOD of each of the at least three different dilutions comprises correlating a current or voltage measurement to a BOD value.
5. The method of claim 3 , wherein at least five different dilutions of the sample are obtained and measured for BOD.
6. The method of claim 3 , wherein the method is completed in less than 1 hour.
7. The method of claim 3 , further comprising determining whether one or more cells in the bioelectrochemical system is operating in a linear range, saturated, or below threshold, and collecting measurements in the linear range.
8. The method of claim 5 , further comprising determining whether one or more cells in the bioelectrochemical system is operating in a linear range, saturated, or below threshold, and collecting measurements in the linear range.
9. A sensor for determining the presence of targeted organic compounds, comprising:
first and second electrodes, configured to operate under anaerobic conditions;
a voltage source operably coupled to the first and second electrodes and configured to provide a voltage;
a current sensor operably coupled to the first and second electrodes and capable of measuring a current between the first and second electrodes;
a culture of exoelectrogenic bacteria for metabolically utilizing the targeted organic compounds as an electron donor; and
a housing for the first and second electrodes, wherein the housing limits aerobic metabolism in proximity to the first electrode and the second electrode.
10. The sensor of claim 9 , wherein the culture comprises Geobacter sulfurreducens .
11. The sensor of claim 9 , wherein the targeted organic compounds comprise a volatile fatty acid.
12. The sensor of claim 9 , further comprising a processor operably connected to the current sensor, capable of receiving a value of current and determining a value of biological oxygen demand from the value of current.
13. The sensor of claim 12 , wherein the sensor is operable to output a signal to a process control system for optimizing performance of an anaerobic system, the system comprising an anaerobic digester, and the processor is operably connected to the process control system and capable of initiating changes in the operation of the anaerobic digester in response to changes in the current.
14. The sensor of claim 9 , wherein one of the first or second electrodes comprises stainless steel.
15. A sensor for determining the presence of targeted organic compounds, comprising:
first and second electrodes, configured to operate under anaerobic conditions;
a voltage source operably coupled to the first and second electrodes and configured to provide a voltage;
a current sensor operably coupled to the first and second electrodes and capable of measuring a current between the first and second electrodes;
a culture of exoelectrogenic bacteria for metabolically utilizing the targeted organic compounds as an electron donor, wherein the current resulting from the metabolization of the targeted organic compounds determines the presence of the targeted organic compounds; and
a housing for the first and second electrodes, wherein the housing limits aerobic metabolism in proximity to the first electrode and the second electrode.
16. A electric current or voltage sensor for determining the presence of targeted organic compounds, comprising:
first and second electrodes, configured to operate under anaerobic conditions;
a voltage source operably coupled to the first and second electrodes and configured to provide a voltage;
a current sensor operably coupled to the first and second electrodes and capable of measuring a current between the first and second electrodes;
a culture of exoelectrogenic bacteria for metabolically utilizing the targeted organic compounds as an electron donor, wherein such metabolic activity of the exoelectrogenic bacteria is specific for the targeted organic compounds; and
a housing for the first and second electrodes, wherein the housing limits aerobic metabolism in proximity to the first electrode or the second electrode.
17. The system of claim 1 , wherein the sensor is configured to determine a minimum dilution rate to enhance accuracy.
18. The sensor of claim 9 , wherein the sensor is configured to determine a minimum dilution rate to enhance accuracy.
19. The sensor of claim 9 , wherein the sensor, further comprises:
a buffer chamber.