Herbicide-resistant proteins, encoding genes, and uses thereof
Some embodiments of the present invention can include herbicide-resistant proteins, coding genes, and uses thereof. Certain herbicide-resistant proteins can comprises: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO: 2; or (b) a protein consisting of an amino acid sequence at least 90% identical to that set forth in SEQ ID NO: 2. Other herbicide-resistant proteins of this disclosure can be suitable for expression in plants, can have resistance to herbicides (e.g., to phenoxy auxinherbicides), or both.
1. An herbicide-resistant protein selected from the group consisting of:
(a) a protein encoded by nucleotide sequence set forth in SEQ ID NO: 3;
(b) a protein encoded by nucleotide sequence set forth in SEQ ID NO: 4; and
(c) a protein encoded by nucleotide sequence set forth in SEQ ID NO: 5.
2. An herbicide-resistant gene selected from the group consisting of:
(a) a nucleotide sequence encoding the herbicide-resistant protein of claim 1 ; and
(b) a nucleotide sequence completely complementary with a nucleotide sequence encoding the herbicide-resistant protein of claim 1 .
3. A method for controlling weeds comprising a step of applying an effective amount of one or more herbicides to the field planted with plants comprising the herbicide-resistant gene of claim 2 or a nucleotide sequence encoding SEQ ID NO: 2.
4. A method for controlling weeds comprising a step of applying an effective amount of one or more herbicides to the field planted with plants comprising the herbicide-resistant gene of claim 2 or a nucleotide sequence encoding SEQ ID NO: 2, wherein the herbicide resistant gene of claim 2 or the nucleotide sequence encoding SEQ ID NO: 2 is produced from a transgenic host cell selected from the group consisting of plant cells, animal cells, bacteria, yeast, baculovirus, nematodes and algae.
5. The method for controlling weeds of claim 3 , wherein the plant is selected from the group consisting of soybean, cotton, corn, rice, wheat, beet and sugarcane.
6. A method for controlling weeds comprising a step of applying an effective amount of one or more herbicides to the field planted with plants comprising the herbicide-resistant gene of claim 2 or a nucleotide sequence encoding SEQ ID NO: 2, wherein the herbicide resistant gene of claim 2 or the nucleotide sequence encoding SEQ ID NO: 2 is co-expressed in the plant with at least a second nucleotide sequence encoding a herbicide-resistant protein different from the herbicide-resistant gene of claim 2 and the nucleotide sequence encoding SEQ ID NO: 2.
7. The method for controlling weeds of claim 6 , wherein said second nucleotide sequence encodes glyphosate-resistant protein, glufosinate ammonium resistant protein, 4-hydroxyphenylpyruvate dioxygenase, acetolactate synthase, cytochromes protein or protoporphyrinogen oxidase.
8. The method for controlling weeds of claim 3 , wherein the herbicide is a phenoxy auxin.
9. The herbicide-resistant protein of claim 1 , wherein the nucleotide sequence set forth in SEQ ID NO: 4 is part of a recombinant expression vector.
10. The herbicide-resistant protein of claim 1 , wherein the nucleotide sequence set forth in SEQ ID NO: 4 is part of a recombinant expression vector and the recombinant expression vector comprises a nucleic acid sequence that is heterologous to SEQ ID NO: 4.
11. The herbicide-resistant protein of claim 1 selected from the group consisting of:
(a) a protein encoded by nucleotide sequence set forth in SEQ ID NO: 3; and
(b) a protein encoded by nucleotide sequence set forth in SEQ ID NO: 5.
12. A method for controlling weeds comprising a step of applying an effective amount of one or more herbicides to the field planted with plants comprising the herbicide-resistant gene of claim 2 .