Methods and compositions for diagnosis of thyroid conditions
The present invention relates to compositions, kits, and methods for molecular profiling and cancer diagnostics, including but not limited to genomic DNA markers associated with cancer. In particular, the present invention provides molecular profiles associated with thyroid cancer, methods of determining molecular profiles, and methods of analyzing results to provide a diagnosis.
1. A method for processing or analyzing a fine needle aspirate (FNA) sample from a thyroid tissue of a human subject, comprising:
(a) providing said FNA sample from said thyroid tissue of said human subject, wherein a portion of said FNA sample is subjected to a cytological or histochemical analysis and is identified as indeterminate by said cytological or histochemical analysis;
(b) assaying deoxyribonucleic acid (DNA) molecules derived from a second portion of said FNA sample using probes that bind to sequences corresponding to IYD, to identify a variation in copy number of IYD in said FNA sample based on a detected probe intensity, wherein said variation in copy number is determined relative to a normal sample;
(c) using at least said variation in copy number for IYD identified in (b) to determine that said thyroid tissue of said human subject comprises a cancerous thyroid tumor; and
(d) subsequent to (c), treating said human subject, wherein said treating comprises performing a thyroidectomy on said human subject.
2. The method of claim 1 , wherein the cancerous thyroid tumor is follicular carcinoma, follicular variant of papillary carcinoma, or papillary thyroid carcinoma.
3. The method of claim 1 , wherein said normal sample comprises a biological sample from said human subject.
4. The method of claim 1 , wherein said normal sample comprises a biological sample from a different subject.
5. The method of claim 1 , wherein said cancerous thyroid tumor is identified at a sensitivity greater than 95%.
6. The method of claim 1 , wherein said DNA molecules of said second portion of said FNA sample are assayed using array hybridization.
7. The method of claim 1 , wherein (b) further comprises assaying said DNA molecules derived from said second portion of said FNA sample using probes that bind to sequences corresponding to at least one additional gene selected from PSD4, Clorf68, KPRP, LCE1E, ST3GAL5, PKHD1L1, PDE8B and ZNF610.
8. The method of claim 7 , wherein (b) further comprises assaying said DNA molecules derived from said second portion of said FNA sample using probes that bind to sequences corresponding to at least two additional genes selected from PSD4, Clorf68, KPRP, LCE1E, ST3GAL5, PKHD1L1, PDE8B and ZNF610.
9. The method of claim 8 , wherein (b) further comprises assaying said DNA molecules derived from said second portion of said FNA sample using probes that bind to sequences corresponding to at least three additional genes selected from PSD4, Clorf68, KPRP, LCE1E, ST3GAL5, PKHD1L1, PDE8B and ZNF610.
10. The method of claim 1 , wherein (b) comprises identifying an increase in said detected probe intensity.
11. The method of claim 1 , wherein (b) comprises identifying a decrease in said detected probe intensity.
12. The method of claim 1 , wherein (b) comprises identifying no increase and no decrease in said detected probe intensity, and wherein (c) comprises determining that said thyroid tissue of said human subject comprises a benign thyroid tumor.
13. The method of claim 1 , wherein said DNA molecules are generated at least in part using reverse transcription of ribonucleic acid molecules in said second portion of said FNA sample.
14. The method of claim 1 , further comprising outputting a report that indicates that said thyroid tissue comprises said cancerous thyroid tumor or a benign thyroid tumor.
15. The method of claim 1 , wherein said assaying comprises sequencing said DNA molecules.
16. The method of claim 1 , wherein said probes are primers, and wherein said assaying comprises performing polymerase chain reaction on said DNA molecules using said primers.
17. The method of claim 1 , further comprising monitoring said human subject for a progression of said cancerous thyroid tumor.