Electrokinetic confinement of neurite growth for dynamically configurable neural networks
Systems and methods for altering neurite growth are generally described. In some embodiments, a system may include a neuron comprising a neurite and electrodes able to generate a physical guidance cue. The physical guidance cue may be used to alter the growth of the neurite and may be temporally and spatially dynamic, such that neurite growth may be altered in a spatial and/or temporal manner. Dynamic control of neurite growth may be used to form directional neural connections, intersections, and/or overlaps.
1. A method comprising
providing a first chamber containing one or more first neurites:
providing a second chamber containing one or more second neurites, wherein the first and second chamber are connected by a first channel:
providing an alternating current electric field in the first channel, with the electric field being aligned with at least a portion of the first channel: and
switching on and off the alternating current electric field over time for directionally guiding elongation of neurites inside the first channel by repelling neurite growth in the alternating current electric field region until at least one neurite of the first chamber overlaps at least one neurite of the second chamber.
2. A method according to claim 1 , comprising directionally guiding elongation of a first population of neurites and a second population of neurites to form a neural network between the first and second population of neurites.
3. A method as in claim 2 , wherein the magnitude of the electric field is greater than or equal to about 100 V/m.
4. A method as in claim 2 , wherein the frequency of the electric field is greater than or equal to about 100 Hz.
5. A method as in claim 2 , wherein the electric field is non-uniform.
6. A method as in claim 2 , wherein said populations of neurites are within a three-dimensional scaffold.
7. A method as in claim 6 , wherein the scaffold is a gel matrix.
8. A method as in claim 1 , wherein the overlap occurs within a three-dimensional scaffold.
9. A method as in claim 8 , wherein the scaffold is a gel matrix.
10. A method as in claim 1 , wherein the neurite is an axon.
11. A method as in claim 1 , wherein the second neurite originates from a different neuron than the first neurite.
12. A method as in claim 1 , wherein the alternating current electric field is switched on and off for a duration and over time to cause the first neurite to overlap with the second neurite without forming a neural connection.
13. A method as in claim 1 , wherein at least one neurite originates from a neuron selected from the group consisting of hippocampus neurons, dorsal root ganglion neurons, and retinal ganglion neurons.