Methods for making plants resistant to fungal pathogens
This invention relates to polynucleotide sequences encoding genes that can confer resistance to the plant pathogen Colletotrichum , which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum , and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the genes from corn lines carrying said genes into other corn lines that do not carry said genes, in order to make them resistant to Colletotrichum and resistant to stalk rot.
1. A process of identifying a corn plant that displays enhanced resistance to Colletotrichum infection, the process comprising:
a. detecting in the corn plant the presence or absence of a polynucleotide sequence comprising SEQ ID NO: 255;
b. and selecting the corn plant in which said polynucleotide sequence is present; and
crossing the selected corn plant in which said polynucleotide sequence is present, with a second corn plant lacking said polynucleotide sequence.
2. The process of claim 1 , further comprising detecting the presence or absence of a marker on or within
SEQ ID NO: 137.
3. The process of claim 1 , further comprising detecting the presence or absence of a marker on or within the Rcg1 coding sequence.
4. The process of claim 3 , wherein the Rcg1 coding sequence comprises a nucleotide sequence encoding a polypeptide, wherein the polypeptide has an amino acid sequence of at least 95% identity when compared to SEQ ID NO:3 based on the Needleman-Wunsch alignment algorithm.
5. The process of claim 1 , further comprising screening a segregating population for a member containing said polynucleotide sequence.
6. The process of claim 5 , further comprising selecting the member for further crossing and selection for enhanced resistance to Colletotrichum infection.