Engineered polypeptide agents for targeted broad spectrum influenza neutralization
The present invention provides novel agents for broad spectrum influenza neutralization. The present invention provides agents for inhibiting influenza infection by bind to the influenza virus and/or hemagglutinin (HA) polypeptides and/or HA receptors, and reagents and methods relating thereto. The present invention provides a system for analyzing interactions between infolds and the interaction partners that bind to them.
1. A method comprising steps of:
providing a sample from a patient suspected of suffering from an influenza infection;
contacting the sample with an infold agent that binds a hemagglutinin (HA) polypeptide, wherein the infold agent comprises an infold selected from the group consisting of Infold-1, Infold-2, Infold-3, Infold-4, Infold-5, Infold-6, Infold-7, Infold-8, Infold-9, Infold-10, Infold-11, Infold-12, Infold-13, Infold-14, Infold-15, Infold-16, Infold-17, Infold-18, Infold-19, Infold-20, Infold-21, Infold-22, Infold-23, Infold-24, Infold-25, Infold-26, Infold-27, Infold-28, Infold-29, Infold-30, Infold-31, Infold-32, Infold-33, Infold-34, Infold-35, Infold-36, Infold-37, Infold-38, Infold-39, and Infold-40; and
detecting binding of the infold agent to components in the sample; and
based on the detected binding, diagnosing the patient as suffering from an influenza infection.
2. The method of claim 1 , wherein prior to the step of contacting, the sample is processed by subjecting it to one or more purification steps.
3. The method of claim 1 , wherein the infold agent is labeled with a detectable moiety.
4. The method of claim 3 , wherein the detectable moiety is a fluorescent moiety.
5. The method of claim 3 , wherein the detectable moiety is a radioactive moiety.
6. The method of claim 3 , wherein the detectable moiety is a chemoluminescent moiety.
7. The method of claim 1 , wherein the infold agent is detected by an immunological method.
8. The method of claim 7 , wherein the immunological method is Western blotting.
9. The method of claim 7 , wherein the immunological method is ELISA.
10. The method of claim 7 , wherein the immunological method is immunofluorescence.
11. The method of claim 1 , wherein the infold agent is immobilized.
12. The method of claim 11 , wherein the infold agent is immobilized to a bead.
13. The method of claim 11 , wherein the infold agent is immobilized to a microtiter dish.
14. The method of claim 11 , wherein the infold agent is immobilized to an array.
15. The method of claim 14 , wherein the array is a glycan array.