Anti-CD40 antibodies
The present invention includes compositions and methods for the expression, secretion and use of novel compositions for use as, e.g., vaccines and antigen delivery vectors, to delivery antigens to antigen presenting cells. In one embodiment, the vector is an anti-CD40 antibody, or fragments thereof, and one or more antigenic peptides linked to the anti-CD40 antibody or fragments thereof, including humanized antibodies.
1. An antibody or an antigen binding fragment thereof that binds to CD40, wherein the antibody or an antigen binding fragment comprises light chain complementarity determining regions 1, 2 and 3 of the light chain variable region of SEQ ID NO: 4 or SEQ ID NO: 5 and heavy chain complementarity determining regions 1, 2 and 3 of the heavy chain variable region of SEQ ID NO: 3.
2. The antibody or antigen binding fragment of claim 1 , wherein the antibody or antigen binding fragment comprises the light chain variable region of SEQ ID NO: 4 or SEQ ID NO: 5 and the heavy chain variable region of SEQ ID NO: 3.
3. The antibody or antigen binding fragment of claim 1 , wherein the antibody or antigen binding fragment comprises the light chain of SEQ ID NO: 4 or SEQ ID NO: 5 and the heavy chain of SEQ ID NO: 3.
4. The antibody of claim 1 , wherein the antibody is produced by the 12B4.2C10 hybridoma (ATCC Accession No. PTA-10653).
5. The antibody or antigen binding fragment of claim 1 , wherein the antibody or antigen binding fragment is humanized.
6. The antibody or antigen binding fragment of claim 1 , wherein the antibody or antigen binding fragment is an Fab, Fab′, Fab′-SH, Fv, scFv, F(ab′) 2 , or a diabody.
7. The antibody or antigen binding fragment of claim 1 , wherein the antibody or antigen binding fragment alone is capable of causing dendritic cells to secrete at least one of IL-6, MIP-1a, IL-12p40, or TNFalpha without prior activation of the dendritic cells.
8. A composition comprising the antibody or antigen binding fragment of claim 1 in combination with a pharmaceutically acceptable carrier or diluent.
9. The antibody or antigen binding fragment of claim 1 , wherein the antibody or antigen binding fragment thereof further comprises a gamma-1, gamma-2, gamma-3, or gamma-4 human heavy chain constant region or variant thereof, or a lambda or a kappa human light chain constant region.