Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.
1. A method of producing one or more polypeptides from a modified fungal cell comprising one or more nucleic acids, each nucleic acid comprising at least one coding region encoding at least one polypeptide, wherein the at least one coding region is operably-linked to at least one regulatory region comprising at least one promoter active in said cell, the method comprising the steps of:
(a) culturing said cell in a culture medium under conditions conducive to expression of said one or more polypeptides encoded by at least one nucleic acid comprising at least one coding region operably-linked to at least one regulatory region comprising at least one promoter active in said cell; and
(b) purifying said one or more polypeptides or a complex comprising said one or more polypeptides expressed in step (a) from the cells or from cell-free medium obtained from the culture,
wherein the modified fungal cell:
(i) is a fungal cell selected from the group consisting of:
(A) Chrysosporium strain UV18-25 deposited as VKMF-3631 D,
(B) Trichoderma longibrachiatum strain X-252, and
(C) a derivative or mutant of any of (A)-(B);
(ii) has a deletion of at least one protease gene selected from the group consisting of alp1, alp2, and pep4; and
(iii) has a culture viscosity phenotype wherein said culture viscosity is less than 200 cP at the end of fermentation, when cultured in suspension in the presence of adequate nutrients and under optimal or near-optimal conditions.
2. A method of producing one or more polypeptides from a modified fungal cell comprising one or more nucleic acids, each nucleic acid comprising at least one coding region encoding at least one polypeptide, wherein the at least one coding region is operably-linked to at least one regulatory region comprising at least one promoter active in said cell, the method comprising the steps of:
(a) culturing said cell under conditions conducive to formation of a plurality of transferable reproductive elements in suspension;
(b) separating the suspension of transferable reproductive elements into a liquid or solid medium suitable for the growth of monoclonal cultures or monoclonal colonies;
(c) culturing the monoclonal cultures or monoclonal colonies from step (b) in medium under conditions conducive to expression of said one or more polypeptides encoded by at least one nucleic acid comprising at least one coding region operably-linked to at least one regulatory region comprising at least one promoter active in said cell; and
(d) purifying said one or more polypeptides or a complex comprising said one or more polypeptides from the cells or from cell-free medium obtained from the culture of step (c),
wherein the modified fungal cell:
(i) is a fungal cell selected from the group consisting of:
(A) Chrysosporium strain UV18-25 deposited as VKM F-3631 D,
(B) Trichoderma longibrachiatum strain X-252, and
(C) a derivative or mutant of any one of (A)-(B);
(ii) has a deletion of at least one protease gene selected from the group consisting of Alp1, alp2 and pep4; and
(iii) has a culture viscosity phenotype wherein said culture viscosity is less than 200 cP at the end of fermentation, when cultured in suspension in the presence of adequate nutrients and under optimal or near-optimal conditions.
3. A method of screening a plurality of polypeptides encoded by a combinatorial library of vectors for a functional activity or a structural property of interest in a modified fungal cell, wherein said modified fungal cell comprises one or more nucleic acids, each nucleic acid comprising at least one coding region encoding at least one polypeptide, wherein the at least one coding region is operably-linked to at least one regulatory region comprising at least one promoter active in said cell, which method comprises the steps of:
(a) culturing said cell under conditions conducive to formation of a plurality of transferable reproductive elements in suspension;
(b) separating the suspension of transferable reproductive elements into liquid or solid medium suitable for the growth of monoclonal cultures or monoclonal colonies; and
(c) culturing the monoclonal cultures or monoclonal colonies of (step (b) in medium under conditions conducive to expression of said one or more poiypeptides encoded by at least one nucleic acid comprising at least one coding region operably-linked to at least one regulatory region comprising at least one promoter active in said cell; and
(d) identifying the presence or measuring the amount of said polypeptides or a complex comprising said one or more polypeptides obtained from the cells or from cell-free medium obtained from said culture of step (c),
wherein said modified fungal cell:
(i) is a fungal cell selected from the group consisting of:
(A) Chrysosporium strain UV18-25 deposited as VKMF-3631 D,
(B) Trichoderma longibrachiatum strain X-252, and
(C) a derivative or mutant of any of (A)-(B);
(ii) has a deletion of at least one protease gene selected from the group consisting of alp1, alp2 and pep4; and
(iii) has a culture viscosity phenotype wherein said culture viscosity is less than 200 cP at the end of fermentation, when cultured in suspension in the presence of adequate nutrients and under optimal or near-optimal conditions.
4. The method of claim 3 , wherein step (d) is a high-throughput screening step.
5. The method of claim 3 , wherein step (d) comprises one of the following techniques: Western blot, immunoblot, enzyme-linked immunosorbant assay, radioimmunoassay, immunoprecipitation, surface plasmon resonance, chemiluminescence, fluorescence polarization, phosphorescence, immunohistochemical analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, microcytometry, microscopy, flow cytometry, protein microchip, protein microarray analysis, or a cell-based bioassay.
6. The method of claim l, wherein the fungal cell is a derivative of strain UV18#100f and is selected from the group consisting of:
UV18#100f Δalp1,
UV18#100f Δpyr5 Δalp1,
UV18#100.f Δalp1Δpep4Δalp2,
UV18#100.f Δpyr5 Δalp1Δpep4 Δalp2, and
UV18#100.f Δpyr4 Δpyr5 Δalp1Δpep4 Δalp2.
7. The method of claim 1 , wherein the fungal cell has been modified to have less than 10% of the protease activity of a parental or non-modified fungus.
8. The method of claim 1 , wherein the fungal cell has been modified to have less than 1% of the protease activity of a parental or non-modified fungus.
9. The method of claim 1 , wherein the culture viscosity phenotype of the fungal cell is less than 100 cP.
10. The method of claim 1 , wherein the culture viscosity phenotype of the fungal cell is less than 60 cP.
11. The method of claim 1 , wherein the culture viscosity phenotype of the fungal cell is less than 10 cP.
12. The method of claim 1 , wherein the fungal cell has been modified to have less than 50% of the protease activity of a parental or non-modified fungus.
13. The method of claim 2 , wherein the fungal cell has been modified to have less than 50% of the protease activity of a parental or non-modified fungus.
14. The method of claim 3 , wherein the fungal cell has been modified to have less than 50% of the protease activity of a parental or non-modified fungus.
15. The method of claim 1 wherein the modified fungal cell is Chrysosporium strain UV18-25 deposited as VKMF-3631 D or a derivative or mutant thereof.
16. The method of claim 2 wherein the modified fungal cell is Chrysosporium strain UV18-25 deposited as VKMF-3631 D or a derivative or mutant thereof.
17. The method of claim 3 wherein the modified fungal cell is Chrysosporium strain UV18-25 deposited as VKMF-3631 D or a derivative or mutant thereof.