Flow cell array and uses thereof
Apparatus and methods for using a flow cell array are provided herein. An apparatus includes an array comprising one or more pixels, wherein each of the one or more pixels comprises multiple reaction sites openings; a first set of one or more sub-surface channels, wherein each of the multiple reaction site openings is connected to a sub-surface channel from the first set of one or more sub-surface channels; a second set of two or more sub-surface channels; and multiple vias connecting each channel from the first set of one or more sub-surface channels to (i) a first sub-surface channel from the second set of two or more sub-surface channels and (ii) a second sub-surface channel from the second set of two or more sub-surface channels.
1. An apparatus comprising:
an array comprising multiple pixels, wherein each of the pixels comprises multiple reaction sites;
a network comprising (i) a set of two or more surface channels each aligned in a first direction within the array, and (ii) at least two sets of two or more sub-surface channels each aligned in a second direction within the array, the second direction perpendicular to the first direction, wherein a first of the at least two sets comprises a chemistry delivery site, wherein a second of the at least two sets comprises a chemistry removal site, and wherein each of the surface channels:
(i) contains one or more iterations of a single reaction site from the multiple reaction sites, and
(ii) is exclusively connectable to (a) a distinct sub-surface channel in the first of the at least two sets and (b) a distinct sub-surface channel in the second of the at least two sets, such that each of the multiple reaction sites is exclusively connectable to a distinct chemistry delivery site and a distinct chemistry removal site; and
multiple vias connecting each of the surface channels to (i) the the corresponding distinct sub-surface channel in the first of the at least two sets and (ii) the the corresponding distinct sub-surface channel in the second of the at least two sets.
2. The apparatus of claim 1 , wherein a size of each of the pixels comprises at least one micrometer.
3. The apparatus of claim 1 , wherein each of the pixels comprises an equal number of reaction sites.
4. The apparatus of claim 1 , wherein location of the reaction sites on each of the pixels is determined based on one or more Gauss numbers.
5. The apparatus of claim 1 , wherein:
said surface channels are parallel and do not overlap;
said sub-surface channels in each of the at least two sets are parallel and do not overlap; and
said surface channels run perpendicular to said at least two sets of sub-surface channels.