Method for allogeneic cell therapy
A method of manipulating allogeneic cells for use in allogeneic cell therapy providing a composition of highly activated allogeneic T-cells which are infused into immunocompetent cancer patients to elicit a novel anti-tumor immune mechanism, or “Mirror Effect”. In contrast to current allogeneic cell therapy protocols where T-cells in the graft mediate the beneficial graft vs. tumor (GVT) and detrimental graft vs. host (GVH) effects, the allogeneic cells of the present invention stimulate host T-cells to mediate the “mirror” of these effects. The mirror of the GVT effect is the host vs. tumor (HVT) effect. The “mirror” of the GVH effect is the host vs. graft (HVG) effect The anti-tumor HVT effect occurs in conjunction with a non-toxic HVG rejection effect. The highly activated allogeneic cells of the invention can be used to stimulate host immunity in a complete HLA mis-matched setting in a patient.
1. A method of allogeneic transplant into a host that provides an anti-tumor effect without GVHD toxicity to the host, the method comprising:
infusing into the host allogeneic cells of which at least a portion are
activated T-cells derived from a tissue mis-matched normal donor and wherein said allogeneic cells are rejected by the host.
2. The method of claim 1 wherein at least a portion of the allogeneic T-cells are Th1 cells.
3. The method of claim 1 wherein the allogeneic T-cells are activated by cross-linking CD3 and CD28 surface antigens.