Methods and compositions for tissue regeneration
A kit is disclosed that includes a first component comprising alginate, wherein the first component is comprised in a first sterile vile, and a second component comprising cells comprising keratinocytes or fibroblasts, or mixtures thereof, that secrete one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ, angiopoietin, EGF, IL-Iβ, TGFα, and TNFα, wherein the cells are allogeneic and mitotically inactive, a buffered solution, and human serum albumin or a cryoprotectant, wherein the second component is comprised in a second vial.
1. A composition comprising mitotically inactive autologous or allogeneic cells that are capable of secreting one or more biologically active molecules selected from GM-CSF, VEGF, KGF, aFGF, bFGF, TGFβ, angiopoietin, EGF, IL-1β, IL-6, IL-8, PDGF, HGF/SF, IGF-1, CSF-1, TGFα, and TNFα, wherein the composition is comprised in a buffered solution, a wound dressing, or an extracellular matrix or matrix material.
2. The composition of claim 1 , wherein the cells are allogeneic.
3. The composition of claim 1 wherein the cells are mitotically inactivated by irradiation.
4. The composition of claim 3 , wherein the cells are mitotically inactivated by irradiation with gamma rays, X-rays, or UV light.
5. The composition of claim 1 , wherein the cells are mitotically inactivated by administration of chemically-based mitotic inhibitors.
6. The composition of claim 1 , wherein the cells are differentiated.
7. The composition of claim 1 , wherein the concentration of cells are in the range of about 1×10 3 cells/μl to about 50×10 3 cells/μl.
8. The composition of claim 1 , wherein the composition is comprised in a buffered solution.
9. The composition of claim 8 , wherein the buffered solution is HBSS.
10. The composition of claim 9 , wherein the HBSS comprises Ca++ and Mg++.
11. The composition of claim 1 , wherein the cell preparation is comprised in a wound dressing.
12. The composition of claim 1 , wherein the composition is comprised in an extracellular matrix or matrix material.
13. The composition of claim 1 , wherein the composition is in the form of a paste.
14. The composition of claim 1 , wherein the composition is in the form of a spray.
15. The composition of claim 1 , further comprising a cryoprotectant.
16. The composition of claim 15 , wherein the composition is cryopreserved.
17. The composition of claim 1 , wherein the composition is comprised in a container.
18. The composition of claim 1 , wherein the composition is comprised in a sterile vial.
19. A method of administering the composition of claim 1 to a wound, the method comprising administering the composition to the wound.
20. A method of storing the composition of claim 1 , the method comprising placing the composition in a container and storing the composition.