Polynucleotides encoding BSL2v2c2-Ig
The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.
1. An isolated polynucleotide comprising a sequence selected from the group consisting of:
(a) an isolated polynucleotide comprising nucleotides encoding amino acids 1 to 480 of SEQ ID NO:135;
(b) an isolated polynucleotide comprising nucleotides encoding amino acids 2 to 480 of SEQ ID NO:135; and
(c) an isolated polynucleotide comprising nucleotides encoding amino acids 29 to 480 of SEQ ID NO:135.
2. The isolated polynucleotide of claim 1 , wherein said polynucleotide is (a).
3. The isolated polynucleotide of claim 2 , wherein said polynucleotide comprises nucleotides 1 to 1440 of SEQ ID NO:134.
4. The isolated polynucleotide of claim 1 , wherein said polynucleotide is (b).
5. The isolated polynucleotide of claim 4 , wherein said polynucleotide comprises nucleotides 4 to 1440 of SEQ ID NO:134.
6. The isolated polynucleotide of claim 1 , wherein said polynucleotide is (c).
7. The isolated polynucleotide of claim 6 , wherein said polynucleotide comprises nucleotides 85 to 1440 of SEQ ID NO:134.
8. An isolated polynucleotide comprising the cDNA clone contained in plasmid BSL2v2c2Ig in ATCC Deposit No. PTA-4056.
9. A recombinant vector comprising the isolated polynucleotide of claim 1 .
10. An isolated recombinant host cell comprising the vector of claim 9 .
11. A method of making an isolated polypeptide comprising:
(a) culturing the recombinant host cell of claim 10 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.
12. The isolated polynucleotide of claim 1 wherein said nucleic acid sequence further comprises a heterologous nucleic acid sequence.
13. The isolated polynucleotide of claim 12 wherein said heterologous nucleic acid sequence encodes a heterologous polypeptide.
14. An isolated polynucleotide comprising nucleotides encoding the extracellular domain of SEQ ID NO:135 fused to a heterologous nucleic acid sequence encoding the Fc domain of an immunoglobulin.
15. The isolated polynucleotide according to claim 14 wherein said extracellular domain is amino acids 1 to 247 of SEQ ID NO:135.
16. An isolated polynucleotide comprising nucleotides encoding the mature extracellular domain of SEQ ID NO:135 fused to a heterologous nucleic acid sequence encoding the Fc domain of an immunoglobulin.
17. The isolated polynucleotide according to claim 16 wherein said mature extracellular domain is amino acids 29 to 246 of SEQ ID NO:135.
18. An isolated polynucleotide comprising a sequence selected from the group consisting of:
(a) an isolated polynucleotide encoding a fusion protein comprising a first nucleic acid sequence encoding amino acids 1 to 246 of SEQ ID NO:135 fused to a second nucleic acid sequence encoding the Fc domain of an immunoglobulin;
(b) an isolated polynucleotide encoding a fusion protein comprising a first nucleic acid sequence encoding amino acids 2 to 246 of SEQ ID NO:135 fused to a second nucleic acid sequence encoding the Fc domain of an immunoglobulin; and
(c) an isolated polynucleotide encoding a fusion protein comprising a first nucleic acid sequence encoding amino acids 29 to 246 of SEQ ID NO:135 fused to a second nucleic acid sequence encoding the Fc domain of an immunoglobulin.
19. The isolated polynucleotide of claim 18 , wherein said first nucleic acid sequence of (a) comprises nucleotides 1 to 738 of SEQ ID NO:134.
20. The isolated polynucleotide of claim 18 , wherein said first nucleic acid sequence of (b) comprises nucleotides 4 to 738 of SEQ ID NO:134.
21. The isolated polynucleotide of claim 18 , wherein said first nucleic acid sequence of (c) comprises nucleotides 85 to 738 of SEQ ID NO:134.
22. The isolated polynucleotide according to claims 14 , 15 , 16 , 17 , 18 , 19 , 20 , or 21 , wherein said immunoglobulin is IgG 1 .
23. An isolated polynucleotide comprising nucleotides encoding the mature extracellular domain of SEQ ID NO:135.
24. The isolated polynucleotide according to claim 23 , wherein said mature extracellular domain comprises amino acids 29 to 246 of SEQ ID NO:135.
25. The isolated polynucleotide of claim 24 , wherein said polynucleotide comprises nucleotides 85 to 738 of SEQ ID NO:134.